应用响应面法优化金丝桃素的纯化工艺

Optimized purification process of hypericin by response surface methodology

在单因素试验的基础上,根据中心组合试验设计原理,选择上柱液中金丝桃素的浓度、洗脱液中乙醇的浓度和洗脱液用量为考察因素,以产物中金丝桃素含量为响应值,采用3因素5水平的响应面分析法对NKA-9型大孔树脂分离纯化贯叶连翘中金丝桃素的工艺条件及参数进行优化.结果表明:金丝桃素的最佳纯化工艺条件为上柱液中金丝桃素的浓度37.64mg/L,洗脱液乙醇的浓度69.42%,洗脱液的用量12.3BV,此时产物中金丝桃素含量理论值可达到1.42%.经验证试验表明,产物中金丝桃素含量为1.45%,与理论值相符.

To study the technological parameters of the purification process of hypericin in Hypericum perforatum with NKA-9 type macroreticular resin by response surface methodology（RSM）.On the basis of one-factor tests and central composite design principle,independent variables were sample concentration,eluent concentration and volume,while dependent variable was content of hypericin in product.The optimum conditions were evaluated with the analysis of 3 factors and 5 levels and optimized using response surface methodology.The results showed that high yields of hypericin were obtained by this purification technology.Hypericin concentrationon,eluent concentrationon and volume were the major significant parameters on hypericin purification as evaluated.The purification conditions were further investigated with central composite design.The results indicated that the optimal parameters for this method were as hypericin concentrationon at 37.64 mg/L,eluent concentration at 69.42%,eluent volume at 12.3 BV.Under the optimized condition,the maximum productivity of hypericin reached 1.45%.It is suggested that RSM can be used optimize the purification parameters of hypericin,and the optimum mathematic model is highly predictive.