摘要
研究目的：通过分析骨骼肌神经肌肉接头（NMJ）N乙酰胆碱受体（nAChR）亚基在成长期（离乳-性成熟）的表达特征,了解NMJ发育过程中的变化及运动对这一过程的影响。研究方法：对3周龄SD雄性大鼠进行运动增强（跑台运动）和运动减弱（尾部悬吊）干预,测定γ-、ε-、-δnAChR mRNA在3~8周龄的表达水平。结果：1）大鼠腓肠肌γ-、ε-、-δnAChR的表达水平在出生后3~5周迅速下降,至5周龄时仅有微量的表达;2）悬吊组-γnAChR在5周龄时表达水平高于对照组;运动组-γnAChR在8周龄时表达水平低于对照组;3）ε-和δ-nAChR表达水平在组间无显著性差异。结论：骨骼肌NMJ的发育在出生后早期具有典型的敏感期;运动训练可以促进胚胎型-γnAChR的消退,限制运动则显著延缓这一过程;成熟后的NMJ具有一定的结构稳定性。
Objective：To describe the development process of skeletal neuromuscular junction（NMJ） in growth stage as well as the effects of activities in this process through the analysis of N acetylcholine receptor（nAChR） subunits expressions.Methods：3-week-old male SD rats have undergone activity increasing（treadmill training,group T） and activity decreasing（tail suspension,group H） intervention respectively.The expressions of γ-,ε-,δ-nAchR mRNA were detected during 3~8 weeks after birth.Results：1） γ-,ε-,δ-nAChR expressions in rat gastrocnemius decreased rapidly in 3~5 weeks after birth and only presented trace amounts of expression at 5-week-old stage;2） the expression of γ-nAChR was higher in group H at 5-week-old stage and lower in group T at 8-week-old stage compared with control group.There were no significant differences of ε-and δ-nAChR expressions between groups.Conclusion：The development of NMJ in postnatal stage has a typical sensitive period;exercise training can promote the recession of embryonic γ-nAChR,restriction on movements can significantly delay this process instead.The NMJ has certain structural stability after getting mature.
出处
《体育科学》
CSSCI
北大核心
2011年第11期56-60,共5页
China Sport Science
基金
国家体育总局体育科学研究所基本科研业务费课题(2006-17)的部分研究成果
