桂郁金提取物对人肝星状细胞增殖、凋亡影响的研究

Study of Guangxi Curcuma Extract on the Proliferation and Apoptosis in Human Hepatic Stellate Cells

目的:观察桂郁金提取物对人肝星状细胞的作用,了解其对该细胞的增殖和凋亡的影响。方法:将人肝星状细胞株(HSC-LX2)与药物孵育48h后,用MTT比色法测定细胞的增殖抑制率。用流式细胞术(ANNEXIN V-PE/7-AAD双染色法)检测细胞凋亡各阶段的情况。结果:低、中、高浓度桂郁金提取物均可抑制HSC-LX2细胞的增殖,48h的增值抑制率分别为15.4%、49.1%、58.3%;48h的IC50为45μmol/L;均可诱导细胞发生早期及晚期凋亡,48h的总凋亡细胞百分比分别为20.7%、29.8%、34.4%。结论:桂郁金提取物具有抑制HSC-LX2细胞增殖、诱导细胞发生凋亡的能力,从而可以干预肝纤维化病程;以上能力均具有剂量依赖性;且中、高剂量桂郁金提取物组以上能力明显比秋水仙碱组和复方鳖甲软肝片组强。

Objective ： To observe the effects of Guangxi curcuma extract on human hepatic stellate cells and to learn its impacts on the cell proliferation and apoptosis. Methods ： To incubate the human hepatic stellate cells （ HSC - LX2） with the drugs for 48h. Then to measure the cell proliferation inhibition rate by MTF,to detect the situation in different apoptosis phase by flow cytometty （ ANNEXIN V - PE/7 - AAD double staining）. Results ： Low, medium and high concentrations of Guangxi curcuma extract can inhibit proliferation of HSC- LX2 cells. The inhibition rates in 48h were 15.4% ,49.1% ,58.3% respectively;ICS0 in 48h was 45 ~mol / L. All concentrations can induce early and late apoptosis of HSC - LX2 ceils. The percentages of total apoptosis cells in 48h were 20.7% ,29.8% ,34.4%. Conclusion ： Guangxi curcuma extract can inhibit the proliferation of HSC - LX2 cells, induce apoptosis so as to interfere with course of the liver fibrosis. This shows the dose - dependent effect and these effects of Guangxi curcuma extract in middle and high - dose groups were significantly stronger than that of colchicine group and shell liver softenin~ tablets group,