摘要
目的:建立注射用益气复脉(冻干)总木脂素的含量测定方法。方法:采用Phenomenex luna C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-水梯度洗脱;柱温30℃;检测波长218nm;进样量10μL;流速1mL/min。结果:五味子醇甲进样量在0.096～0.578μg范围内线性关系良好,其回归方程为:Y=404589x-9426.4,r=0.9998,精密度RSD1.88%,稳定性RSD2.29%,重复性试验RSD1.49%,平均回收率为99.84%、RSD 2.08%。结论:实验结果表明该方法简便,灵敏,专属性强,重复性好,可作为注射用益气复脉(冻干)的质量控制方法。
Objective ： To establish a HPLC method of the determination of lignanoid in Yiqifumai injection. Methods ： The con- teat of lig naaoid was determined by Phenomenex luna C18 （250 mm×4.6 mm, 5μm） column, the mobile phase was consisted of acetonitrile -water ,the temperature of column was 30℃, the detection wavelength was at 218 nm, the flow rate was 1.0 mL/ min. Results ： The calibration curve of lignanoid was linear in the range of 0.096 - 0.578 μg, the linear equation was Y = 404589x -9426.4,r =0. 9998. The precision（RSD） was 1.88% （n =6）. The reproducibi]ity（RSD） of this method was 1.49% （n =6）. The average recovery of schizandrolA was 99.84% （ RSD 2.08% , n = 6）. Conclusion ： The method is easy, sensitive, specific, and accurate with good reproducibility. It can be used for quality control of lignanoid in Yiqifumai injection.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第11期2239-2241,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
重大新药创制课题(2010ZX09602-004)
科技创新专项资金(08FDZDSH01404)
