应激对大鼠自身免疫性脑脊髓炎模型的影响

Influence of stress on SD rats model of experimental autoimmune encephalomyelitis

目的:观察应激刺激对实验性自身免疫性脑脊髓炎(EAE)SD大鼠细胞免疫的影响。方法:用豚鼠脊髓抗原免疫SD大鼠,制作SD大鼠EAE模型,并随机分为抗原免疫+应激处理组(A组)和单纯抗原免疫组(B组),观察两组大鼠EAE的发病率及病情严重程度。3周后处死SD大鼠,检测大鼠外周血CD4+、CD8+细胞比例和CD4+/CD8+比值。结果:接种豚鼠脊髓抗原的两组大鼠10d后开始发病,A组大鼠EAE发病率和病情严重评分均高于B组,差异有统计学意义(P<0.01)。A组外周血CD4+比例、CD4+/CD8+比值均低于B组,差异有统计学意义(P<0.05),CD8+细胞比例与B组相当,差异无统计学意义(P>0.05)。结论:应激刺激对EAE大鼠细胞免疫有抑制作用,促发并加重大鼠EAE病情。

Objective： To investigate the influence of stress on rat model of experimental autoimmune encephalomyelitis（EAE）.Methods： SD rat models of EAE were induced by injection of guinea-pig spinal cord homogenate,then randomly divided into stress group（group A） and simple homogenate group（group B）,which were intervened with different methods.Severity and incidence of EAE of the two groups were observed and compared.Rats were sacrificed after 3 weeks,levels of CD4^＋,CD8^＋ cells,and CD4^＋/CD8^＋ were detected.Results： Onset of EAE was observed in both groups 10 d after injection of guinea-pig spinal cord homogenate,but more severe and higher incidence was observed in group A（P〈0.05）.The count of CD4^＋ cells and CD4^＋/CD8^＋ ratio in group A was significantly lower than that in group B（P〈0.05）.But no significant difference in CD8^＋ count was observed between the two groups.Conclusions： Interference of stress can induce immunosupression and promote progress of EAE in rats.