红花檵木叶片花色素提取及其性质研究

Extraction and Characterization of Anthocyanidins from Loropetalum chinense var.rubrum Yieh Leaves

以红花檵木叶片为材料,对其花色素的提取条件及理化性质进行研究。结果表明,红花檵木叶片花色素提取的最佳条件为:99.5%的甲醇、料液比1:5(g/mL)、浸提温度60℃、浸提时间2h。红花檵木叶片花色素具光、热不稳定性;在强酸性时稳定,微酸近中性时变色。色素抗氧化、还原能力差;对螯合剂、苯甲酸钠敏感。葡萄糖、蔗糖对色素无明显影响,食盐、柠檬酸有增色作用,VC具减色作用。金属离子A13+、Ca2+、Co2+等具增色作用,Cu2+、Mg2+、Zn2+、Mn2+、K+、Sn2+等有减色作用,Fe2+、Fe3+、Pb2+影响色素稳定性。

The anthocyanidinsins in Loropetalum chinense var.rubrum Yieh leaves were extracted by solvent extraction method and analyzed for their physico-chemical properties.The best extraction conditions were found as follows： 99.5% methanol as extraction solvent at a solid-to-liquid ratio of 1：5（g/mL） for 2 h extraction at 60 ℃.The pigments were unstable to light and heating but stable to strongly acidic environments and had a different color in slightly acidic and nearly neutral environments.Their tolerance to H2O2 and Na2SO3 was poor and their sensitivity to EDTA and sodium benzoate was high.Glucose and sucrose had little effect on the pigments.Salt,citric acid and some metal ions such as A13＋,Ca2＋ and Co2＋ had hyperchromic effect on them and vitamin C and other metal ions such as Cu2＋,Mg2＋,Zn2＋,Mn2＋,K＋ and Sn2＋ had hypochromic effect.Moreover,Fe2＋,Fe3＋ and Pb2＋ affected the stability of the pigments.