AccQ·Tag柱前衍生反相-高效液相色谱法测定齿果酸模中的氨基酸含量

Determination of Amino Acid Contents in the Whole Plant of Rumex dentatus by Reversed-Phase High-Performance Liquid Chromatography with AccQ·Tag Pre-column Derivatization Method

为研究齿果酸模中氨基酸的种类和含量,探讨其营养特性和食用开发价值。以6-氨基喹啉-N-羟基琥珀酰亚胺基甲酸酯(AQC)为衍生试剂,以磷酸盐缓冲液为流动相A,乙腈溶液为流动相B进行梯度洗脱;在柱温37℃条件下用AccQ·Tag进行柱前衍生,反相-高效液相色谱法用紫外检测器在波长248nm进行测定。结果表明:齿果酸模中含有17种氨基酸,干基总含量为252.35mg/g,有很好的食用开发前景。17种氨基酸线性回归方程的相关系数r在0.9995以上(n=5),测定的平均回收率为97.1%～102.6%,相对标准偏差RSD为0.3%～3.2%(n=3),测定结果令人满意。

Objective： To determine the composition and contents of amino acids in the whole plant of Rumex dentatus for further study of its nutritional characteristics and food value.Methods： Amino acids were derivatized by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate（AQC）,and then their derivatives were separated on a Waters 515 HPLC system with a column temperature of 37 ℃ using phosphate buffer as mobile phase A and aqueous acetonitrile as mobile phase B in the gradient elution mode and detected at 248 nm using a UV detector.Results： Totally 17 amino acids were found,and the total content of amino acids in samples was 252.35 mg/g.The correlation coefficients of the calibration curves for 17 amino acids were all above 0.9995（n = 5）.The average spike recoveries of 17 amino acids were between 97.1% and 102.6% with a relative standard deviation（RSD） between 0.3% and 3.26%（n = 3）.