野牛草种质基于SRAP标记的遗传多样性研究

Genetic diversity of Buchloe dactyloides based on SRAP markers

野牛草(Buchloe dactyloides)是一种低养护的暖季型草坪草,本研究利用相关序列扩增多态性(SRAP)标记对来自美国、加拿大和日本的31份野牛草材料进行遗传多样性分析。结果表明,用19对引物组合共扩增出271条多态性条带,多态性位点百分率为86.86%,材料间的遗传相似系数(GS)为0.586 5～0.916 7,平均GS值为0.669 1。两个材料在不同的引物组合扩增下,分别出现特异性条带和缺失性条带,这些条带可用于野牛草基因型的鉴定。非加权成对算术平均法(UPGMA)聚类分析和主成分分析结果表明,供试材料可聚成两类,而相同地理来源的材料并未聚为一类。

Buchloe dactyloides is a warm-season grass for low-maintenance lawns. In this paper, sequencerelated amplified polymorphism （SRAP） molecular markers were used to detect the genetic diversity of 31 accessions of B. dactyloides collected from America, Canada and Japan. Nineteen primer pairs were used to amplify among the accessions. A total of 271 （86.86%） bands were observed. The genetic diversity coefficients （GS） varied from 0. 586 5 to 0. 916 7 among these accessions, with an average of 0. 669 1. Two materials showed the specific and specific deletion bands respectively in the amplified products of different primer combinations. These bands can be used to identify genotypes of B. dactyloides. The dendrogram and principal component analysis revealed from these markers clustered the materials into two main groups. But materials from the same geographic origins were not classified into the same group.