野生马蹄金花粉生活力检测方法比较

Detection methods of pollen viability of wild Dichondra repens

以野生马蹄金(Dichondra repens)材料SD200303和GD200503为对象,分别以碘-碘化钾染色法、联苯胺-甲萘酚染色法、TTC法和离体萌发法测定其花粉活力,比较各检测方法测定效果。结果表明,碘-碘化钾染色法、TTC法染色效果不佳,不适宜野生马蹄金花粉活力测定;联苯胺-甲萘酚染色法能测定花粉生活力,但测定值偏高;离体萌发法测定野生马蹄金花粉活力,花粉管生长良好,且萌发率基本稳定,便于快速直观统计花粉生活力,适宜野生马蹄金花粉生活力检测,其较适宜的培养基为蔗糖10%+硼酸(HBO3)0.005%+硝酸钙[Ca(NO3)2.4H2O]0.015%+硫酸镁(MgSO4.7H2O)0.001%+硝酸钾(KNO3)0.005%+0.5%琼脂。

The pollen viability of wild Dichondra repent＂ ods of I2-KI, 4, 4＇-diaminobiphenyl-l-Naphthol, TTC comparison of the four methods indicated that the meth wild Dichondra repens pollen activity. 4,4＇-diaminobi （SD200303 and GD200503） was detected by methand pollen germination in vitro, respectively. The ods of I2-KI and TTC were not suitable to evaluate phenyl-l-Naphthol method could detect the pollen viability, but the measured value was higher than that of pollen viability. The method of germination in vitro was the fittest and convenient, in which pollen tube grew well and pollen viability was stable. The optimum culture medium was 10% Sucrose＋0.005% HBO3 ＋0. 015% [Ca（NO3）2 4H20] ＋0.001% MgSO4 7 H2 O＋ 0. 005 % KNO3 ＋ 0.5 % Agar.