肺炎支原体感染患者外周血T淋巴细胞亚群变化规律研究

Research on variation about blood peripheral T lymphocyte subsets for patients with Mycoplasma pneumoniae infection

目的探讨肺炎支原体感染患者外周血T淋巴细胞亚群变化规律。方法选择2009年10月-2010年5月收治的34例肺炎支原体感染患者,采用单克隆抗体-碱性磷酸酶抗碱性磷酸酶法(APAAP法)进行急性期和恢复期外周血T淋巴细胞亚群检测,同时以同期于医院健康体检的32名健康者作为对照组,对其进行比较研究。结果与健康者比较,肺炎支原体感染患者外周血急性期、恢复期CD4+分别为(36.25±5.10)%、(35.97±5.42)%,明显降低,CD8+分别为(30.91±1.14)%、(30.98±1.20)%,明显增高,CD4+/CD8+分别为(1.41±0.42)%、(1.40±0.39)%,也明显下降,组间差异均有统计学意义(均P<0.05)。结论肺炎支原体感染患者外周血CD4+明显降低,CD8+明显增高,CD4+/CD8+也明显下降,外周血T淋巴细胞亚群的检测能够肺炎支原体感染的诊断及治疗提供一定的临床指导信息。

OBJECTIVE To investigate the variation about T lymphocyte subsets for patients with Mycoplasma pneumoniae infection. METHODS A total of 34 patients with M.pneumoniae infection in our hospital from Oct 2009 to May 2010 were selected as the research objects.Monoclonal antibody-alkaline phosphatase anti-alkaline phosphatase method（APAAP method） was used to detect acute and convalescent blood T-lymphocyte subsets,and 32 healthy cases in our hospital were chosen as the control group for comparison. RESULTS Compared with healthy subjects,patients with acute phase and recovery phase of M.pneumoniae infection reduced peripheral blood CD4＋,respectively（36.25±5.10）%,（35.97±5.42）%,CD8＋ increased significantly,respectively（30.91±1.14）%,（30.98±1.20）%,CD4＋/CD8＋ also decreased significantly,respectively（1.41±0.42）%,（1.40±0.39）%,there were differences between groups for statistically significant（P〈0.05）. CONCLUSION Peripheral blood for patients with mycoplasma pneumoniae infection CD4＋ reduces significantly,CD8＋ increases significantly,CD4＋/CD8＋ decreased significantly.Peripheral blood T lymphocyte subsets can offer certain clinical guidance for the detection of Mycoplasma pneumoniae infection on the diagnosis and treatment.