pH值及盐度对副溶血弧菌与霍乱弧菌生长影响的研究

Effects of pH value and salinity on growth of Vibrio parahaemolyticus and Vibrio cholerae

目的研究增菌液pH值及盐度对副溶血弧菌和霍乱弧菌生长的影响,找出副溶血弧菌与霍乱弧菌的最佳生长pH和盐度。方法应用阳性菌株副溶血弧菌和霍乱弧菌,参照国际和国内增菌液的要求,控制增菌液pH值和盐度条件,采用弧菌显色培养基检测副溶血弧菌和霍乱弧菌生长数。结果增菌液pH值7.2、盐度3.2%时最适副溶血弧菌生长,增菌液pH值7.1、盐度1.2%时最适霍乱弧菌生长。结论增菌液pH值和盐度对副溶血弧菌和霍乱弧菌生长有一定影响。

OBJECTIVE To study the effect of different pH value and salinity of broth on the growth of Vibrio parahaemolyticus and Vibrio cholerae,and to find out the best pH value and salinity. METHODS The bacterial counts of Vibrio cholerae and Vibrio paraheamolyticus were detected referring to international and domestic request under differrent pH ralue and salinity concerntration. RESULTS The enrichment broth pH 7.2 and salinity 3.2% were the most suitable condition for the growth of V.parahaemolyticus,pH 7.1 and salinity 1.2% were the most suitable condition to the growth of V.cholera. CONCLUSION pH value and salinity of enrichment broth can influence the growth of V.parahaemolyticus and V.cholerae.