摘要
以野蔷薇(Rosa multiflora)为试材,采用RT-PCR和RACE方法从叶片中获得了一个Mlo同源基因的cDNA全长,命名为RmMlo,GenBank登录号为JF310747。序列分析结果表明,RmMlo基因cDNA全长为1993bp,包含49bp的5′非编码区、243bp的3′非编码区和一个长度为1700bp编码567个氨基酸的开放阅读框。氨基酸序列分析显示其蛋白质的C末端具有两个保守的MLO模体。序列比对和系统进化分析表明,RmMlo与拟南芥等双子叶植物的Mlo亲缘关系较近,相似性达90%以上。半定量RT-PCR分析表明,RmMlo在感病叶片中的表达量最高,在根中不表达,其表达模式与拟南芥的AtMlo有一定差别。
In this research,a full-length cDNA sequence of Mlo gene,named RmMlo(GenBank accession No.JF310747)was obtained from leaves of Rosa multiflora by RT-PCR and RACE.Sequence analysis indicated that RmMlo was 1 993 bp in full length containing a 5'-untranslated region(5'-UTR)of 49 bp,a 3'-UTR of 243 bp,and an opening reading frame(ORF)of 1 700 bp encoding 567 amino acids.Subsequently,amino acid sequence analysis revealed that RmMlo had two conserved MLO motifs in the protein C-terminal.Sequence alignment and phylogenetic analysis showed that RmMlo shared more than90%homology with Mlo from Arabidopsis thaliana and other dicotyledon.Finally,semiquantitative RT-PCR analysis indicated that RmMlo showed the highest expression abundance in infected leaves and no expression in root,which was different from the expression pattern of AtMlo.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第10期1999-2004,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31160403)
公益性行业(农业)科研专项(200903020)
国家‘863’计划子课题(2006AA100109)
云南省科技创新强省计划项目(2007C0004Z)
国家科技支撑计划子课题(2006BAD01A1805)