寒兰杂交及种子离体萌发研究

Hybridization and in Vitro Seed Germination of Cymbidium kanran

以寒兰(Cymbidium kanran)为研究对象,通过人工辅助杂交,检测寒兰不同株系的亲和性与种子离体萌发。结果表明,寒兰株系间、寒兰与春兰(C.goeringii)、墨兰(C.sinenses)杂交易成功,坐果率达100%;寒兰和大花蕙兰(C.hybrid)杂交,正反交差异显著,以寒兰为母本,坐果率为75%,以大花蕙兰为母本,坐果率为0;以寒兰为母本,蝴蝶兰(Phalaenopsis amabilis)为父本,坐果率为0。果龄180～240d的寒兰果实适宜于离体播种,其最适宜的培养基为改良KC培养基。黑暗条件适宜寒兰种子萌发。寒兰根状茎分化芽较适宜的培养基为改良KC+0.1mg·L-16-BA+5mg·L-1NAA。

The experiments were carried out for detecting fruit setting and in vitro seed germination in Cymbidium kanran.The results indicated that it was easy to cross between the lines of C.kanran,between C.kanran and C.goeringii,or C.sinense,according to the fact of 100%fruit setting.The percentage of fruit setting between C.kanran and hybrid Cymbidium depended on which species used as female parent.In the case of C.kanran as female parent,the fruit setting reached 75%,while hybrid Cymbidium as female parent the fruit setting dropped to zero.In the case of C.kanran as female parent and Phalaenopsis amabilis as male parent,no fruit was set.The fruits of C.kanran 180 to 240 d after pollination were the suitable materials for in vitro seed germination.The results also indicated that the optimum basal medium for seed in vitro germination was modified KC,the dark condition was necessary for germination,and the suitable combination of plant growth regulators for growth and differentiation of rhizomes was modified KC＋0.1 mg·L-1 6-BA＋5 mg·L-1 NAA.