CyclinA、C-myc在皮肤瘢痕及瘢痕癌组织中的表达及意义

Expressions and Significance of CyclinA and C-myc in Skin Scar and Skin Scar Carcinoma

目的探讨CyclinA、C-myc在皮肤病理性瘢痕和瘢痕癌组织中的表达及意义。方法以病理性皮肤瘢痕、皮肤瘢痕癌组织为研究对象,以正常皮肤组织为对照。采用免疫组织化学(SP法)分别检测CyclinA、C-myc蛋白的表达,采用核酸分子原位杂交法检测CyclinA mRNA的表达,所有数据输入计算机后运用SPSS16.0软件包进行统计学分析。结果 (1)CyclinA、CyclinA mRNA在正常皮肤和皮肤瘢痕上皮中的表达呈阴性或弱阳性,在瘢痕癌组织中呈强阳性。瘢痕癌组的表达(平均吸光度和阳性面积)与正常皮肤组及皮肤瘢痕组比较,差异均有统计学意义(P<0.01);但正常皮肤组与瘢痕组比较,差异无统计学意义(P>0.05)。(2)C-myc蛋白在正常皮肤中的表达呈弱阳性,在皮肤瘢痕上皮中呈阳性,在瘢痕癌组织中呈强阳性。且两两比较差异均有统计学意义(P<0.05)。(3)相关分析显示,在皮肤瘢痕癌中,CyclinA与CyclinA mRNA(r=0.766,P<0.01)、CyclinA与C-myc蛋白(r=0.804,P<0.01)的表达均呈正相关。结论 (1)CyclinA及其mRNA、C-myc的高表达,可能与皮肤瘢痕癌的发生有关;(2)C-myc蛋白的高表达与皮肤瘢痕上皮癌变有相关性。(3)在瘢痕癌中CyclinA同时存在蛋白水平和基因水平的异常表达。

Objective To study the expressions and significance of CyclinA and C-myc in skin Scar and skin Scar Carcinoma. Methods We compared pathological skin scar tissues and skin scar carcinoma tissues with normalskin tissues. The expression of CyclinA and C-myc proteins were detected by the immu- nohistochemical method of SP. And the expression of CyclinA mRNA was detected by in situ hybridiza- tion. All data were input into the computer and statistically analyzed with SPSS （16. 0） software. Results （1） CyclinA and its mRNA showed strong positive expression in scar carcinoma tissues, negative or weakly positive expression in normal skin epidermis and pathological skin scar epithelium. Compared with normal skin group and skin scar group , the expression （optical density and positive area） of CyclinA and its mRNA in scar carcinoma group were statistically significant （P〈0. （）1）. No statistic differences of their expression wei＇e measured between normal skin epidermis and pathological skin scar epitheliun~ （2） C-myc protein showed strong positive, positive and weakly positive expression in scar carcinoma tissues, pathological skin scar epithelium and normal skin epidermis respectively. Each of two groups was statisti- cally different（P〈0.05）. （3）Correlated analysis showed that there was positive correlation between the expression levels of CyclinA and CyclinA mRNA, CyclinA and C-myc proteins in skin scar carcinoma tis- sues. Conclusion （1）The high expression of CyclinA, CyclinA mRNA and C-myc may be related to the occurrence of skin scar carcinoma. （2）The high expression of C-myc may be related to the cancerization of skin scar epithelium. （3）There was the abnormal expression of CyclinA not only on protein levels, but al- so on gene levels in skin scar carcinoma tissues.