RRM2在上皮性卵巢肿瘤组织中的表达及其与血管生成的关系

Expression of RRM2 and Its Relation with Tumor Vascularization in Epithelial Ovarian Neoplasms

目的探讨核糖核苷酸还原酶小亚基(RRM2)在上皮性卵巢肿瘤中的表达及其与血管生成的关系。方法采用免疫组织化学PV-6000二步法和RT-PCR法,检测RRM2、CD105(Endoglin)在正常卵巢、良性卵巢肿瘤、低度潜在恶性上皮性卵巢肿瘤和上皮性卵巢癌组织中的表达,用CD105(Endoglin)标记微血管密度(MVD),并分析RRM2 mRNA和CD105 mRNA表达的关系。结果 (1)低度潜在恶性上皮性卵巢肿瘤组织(1.586±0.650,66.67%)和上皮性卵巢癌组织(1.870±0.618,69.35%)的RRM2 mRNA表达量和蛋白阳性表达率均高于正常卵巢组织(0.771±0.495,0)和良性卵巢肿瘤组织(0.952±0.601,26.67%)(P<0.05)。(2)低度潜在恶性上皮性卵巢肿瘤组织(2.190±0.512,23.15±4.38)和上皮性卵巢癌组织(2.735±0.636,25.27±6.91)中的CD105 mRNA表达量和MVD均高于正常卵巢组织(0.686±0.637,3.40±1.78)和良性卵巢肿瘤组织(0.763±0.547,12.15±2.29)(P<0.05)。(3)RRM2 mRNA表达量和蛋白阳性表达率在低度潜在恶性组和FIGOⅠ～Ⅱ期卵巢癌组高于正常卵巢组和良性卵巢肿瘤组(P<0.05),在FIGOⅢ～Ⅳ期高于Ⅰ～Ⅱ期(t=-2.370,χ2=5.937,P<0.05)。(4)RRM2 mRNA和CD105mRNA之间表达呈正相关(r=0.713,P<0.05)。结论 RRM2可能参与上皮性卵巢癌发生的早期事件,对上皮性卵巢癌的血管生成可能有一定促进作用,有望成为一个新的早期诊断指标。

Abstract： Objective To investigate the correlation of RRM2 expression with angiogenesis in epithelian ovarian cancer. Methods The mRNA and protein level o{ RRM2 and CDI（15 in 98 ovarian specimens（in- cluding 15 normal, 15 benign , 6 borderline and 62 malignant） were detected by RT-PCR and irnmunohis tochemistry. The relationship between the expressions of two genes was analyzed. Results （ 1 ） The mR- NA level and protein positive rates of RR1VL2 in borderline ovarian neoplasms（1. 586± 0.650, 66. 67 %） and ovarian cancers （l. 870± 1）. 61, 69. 35%）were both higher than those in normal group （0. 771 ± 1）. 495,0） and benign group（0. 952 ± 0.61）1, 26. 67%） （P〈0.05）. （2）The mRNA level and MVD of CD 105 in borderline ovarian neoplasms（2. 190± 0. 512, 23. 15 ±4. 38） and ovarian cancers（2. 735 ± 0. 636, 25.27± 6. 91） were both higher than those in normal group（0. 686± 0. 637,3.40± 1.78） and benign group（0. 763 ± 0. 547, 12.15 ± 2.29） （P〈0.05）. （3） The mRNA level and positive rates of RRM2 were higher in borderline ovarian neoplasms and clinical stage I - Ⅱ than those in normal group and benign group （P〈0.05） ,and higher in clinical stage Ⅲ - Ⅳthan that in stage I - Ⅱ （t = - 2. 370, X^2 = 5. 937, P〈0.05）. （4）Expression of RRM2mRNA was positively correlated with CD105mRNA expression in o varian cancer tissues（r= 0. 713,P〈0.05）. Conclusion RRM2 might have participated the early stage of the origination and progression of epithelian ovarian cancer, and might have encouraged effects on anglogenesis of epithelial ovarian cancer, so it may be another new tumor marker for earlier diagnosis.