广东车八岭自然保护区疑似黑熊爪鞘的分子鉴定

Molecular Identification of Suspected Claw Sheath from Ursus thibetanus in Chebaling National Nature Reserve,Guangdong Province

利用食肉目线粒体Cyt b基因的特异性引物对2010年4月在广东车八岭自然保护区内发现的爪鞘进行了分子鉴定。所测序列与亚洲黑熊(Ursus thibetanus)的同源性最高,达97.2%～99.1%,确定爪鞘来自于亚洲黑熊,为近20 a来首次在车八岭保护区发现的亚洲黑熊存在痕迹。

DNA was direcdy extracted from a claw sheath found in Chebaling National Nature Reserve, then the mtDNA Cyt b gene fragment was amplified and sequenced. Sequences were aligned with the GenBank by BLAST software, which showed the highest homology to the sequences from the black bear （ Ursus thibetanus） , reaching 97.2%-99. 1%. The claw sheath was identified from U. thibetanus. It is the first time we have discovered trace of U. thibetanus appeared in Chebaling National Nature Reserve in recent 20 years.