红花如意丸对雌、孕激素负荷大鼠雌二醇、黄体酮、催乳素的影响

Effects of Honghua Ruyi Pill on the Concentration of Estradiol,Progesterone and Prolactin in Rats Induced by Estrogen and Progesterone Load

目的腹腔注射外源性雌激素配合孕激素,建立下丘脑-垂体-性腺轴功能失调大鼠模型,观察红花如意丸对该模型大鼠垂体泌乳素(PRL)、孕酮(P)、雌二醇(E2)的影响。方法雌性Wistar大鼠70只,随机分为7组,正常组,模型组,红花如意丸低、中、高剂量组(0.156g/kg、0.469g/kg、1.407g/kg)、他莫昔芬组及桂枝茯苓胶囊对照组,每组10只。除正常组大鼠,各组大鼠均腹腔注射雌二醇及黄体酮制备高雌二醇、黄体酮大鼠模型,造模同时灌胃给药。空白对照组和模型组给等体积生理盐水,其余组分别给桂枝茯苓胶囊混悬液0.326g/kg、红花如意丸0.156g/kg、0.469g/kg、1.407g/kg和他莫昔芬0.005g/kg,每日灌胃,共30d。末次给药24h后,用放射免疫方法测定各组大鼠血清雌二醇、黄体酮、催乳素的含量。结果和正常组相比,模型组动物血清雌二醇、垂体泌乳素显著增高(P<0.01);红花如意丸(0.469g/kg、1.407g/kg)、桂枝茯苓胶囊组大鼠垂体泌乳素(PRL)含量均明显低于模型组(P<0.01或P<0.05);红花如意丸(0.469g/kg)能提高模型大鼠血清孕酮(P<0.05);红花如意丸(1.407g/kg)还可降低模型大鼠血清雌二醇含量(P<0.01)。结论红花如意丸可调节雌、孕激素负荷大鼠垂体泌乳素、雌二醇激素水平。

Objective The hypothalamus-pituitary-gonadal axis dysfunction model of rats was established by injecting of exogenous estrogen and progesterone,and observe the effects of Honghua Ruyi Pill on the concentration of Estradiol（E2）,Progesterone（P）and Prolactin（PRL） in this model.Method Totally 70 female Wistar rats were divided into seven groups randomly,normal group,model group,Honghua Ruyi Pill high,medium,low-dose group（0.156g/kg,0.469g/kg,1.407g/kg）,Tamoxifen group and Cinnamon twig and Poria capsule group,with 10 rats in each group.All rats injected with estradiol and progesterone to induce the model of high estradiol and progesterone in addition to the normal group,rats were also gavage modeling.To give the rats corresponding dose 30 days by intragastric administration with the Cinnamon twig and Poria capsules suspension 0.326g/kg,Honghua Ruyi Pill 0.156g/kg,0.469g/kg,1.407g/kg and Tamoxifen 0.005g/kg,control group and model group to the same volume of saline.24h after the last administration,the concentrations of Estradiol（E2）,Progesterone（P） and Prolactin（PRL） were examined in serum rats by enzyme linked immunosorbent assay（ELISA）.Results Compared with normal group,the concentration of estradiol（E2） and Prolactin（PRL） increased significantly（P0.01）.The concentration of Prolactin（PRL） in Honghua Ruyi Pill group（0.469g/kg,1.407g/kg） and Cinnamon twig and Poria capsule group were obviously lower than that in model group（P0.01,P0.05）.Honghua Ruyi Pill（0.469g/kg）could increase markedly the concentration of progesterone（P）（P0.05）and Honghua Ruyi Pill（1.407g/kg）could reduce the concentration of estradiol（E2）（P0.01）.Conclusion Honghua Ruyi Pill can regulate prolactin,progesterone,estradiol hormone levels.