栀子金花丸中栀子苷含量的胶束毛细管电泳法测定

Determination of Geniposide in Zhizi Jinhua Wan by HPCE

目的建立测定栀子金花丸中栀子苷含量的胶束毛细管电泳法。方法采用未涂层弹性融硅石英毛细管柱(60 cm×75μm ID,有效长度52 cm);以25 mmol.L-1硼砂+25 mmol.L-1SDS+4 mmol.L-1磺丁基-β-环糊精+8%(体积分数)甲醇(pH 9.5)为运行缓冲液;分离电压14 kV;重力进样10 s(高度15 cm);检测波长238 nm。以维生素B1为内标。结果栀子苷浓度在21.0～63.0μg.ml-1内线性关系良好(r=0.9999),栀子苷平均加样回收率100.5%,方法精密度(RSD)为0.56%(n=6)。结论该方法简便、快速,结果准确可靠,可用于栀子金花丸的质量控制。

Objective To establish the method for determination of geniposide in Zhizi Jinhua Wan by capillary electrophoresis. Methods The separation was performed on a fused silica capillary of 60 cm×75 μm ID（52 cm of effective length）.25 mmol·L-1Na2B4O7＋25 mmol·L-1SDS＋4 mmol·L-1SBE-β-CD＋8%（V/V）CH3OH（pH 9.5） was selected as the running buffer.The separation voltage was 14 kV,the sample was injected by gravity（10 s,15 cm）,and the detection wavelength was 238 nm. Results The linear range of determination for geniposide was 21.0～63.0 μg·ml-1（r=0.999 9）.The average recovery was 100.5%,precision of the method was 0.56%（RSD,n= 6）. Conclusion The method is convenient,rapid and accurate for the quality control of Zhizi Jinhua Wan.