大鼠胃平滑肌细胞药物模型的改良组织块培养法建立

An Improved Drug Model of Cultured Rat Gastric Smooth Muscle Cells by Tissue-piece Method

目的建立一种新型大鼠胃平滑肌细胞药物模型,更好地研究中药胃肠舒片的作用机制。方法培养SD大鼠胃平滑肌细胞,分为传统方法对照组、传统方法西沙必利组、传统方法胃肠舒片组和改进方法对照组、改良方法西沙必利组、改良方法胃肠舒组,对不同组的细胞给予不同方式药物作用,观察细胞生长情况。结果两种方法培养细胞免疫荧光染色均显示特异性平滑肌肌动蛋白阳性。第三代细胞药物模型构建24 h后细胞浓度比较,传统方法对照组和改良方法对照组间无统计学意义(P>0.05),而传统方法西沙必利组和改良方法西沙必利组间、传统方法胃肠舒片组和改良方法胃肠舒组间细胞浓度均为改良方法组大于传统方法组,有统计学意义(P<0.05)。结论改良方法可以有效地构建大鼠胃平滑肌细胞药物模型,用药后可以获得更多高纯度的胃平滑肌细胞。

ObjectiveTo establish a new drug model of cultured rat gastric smooth muscle cells,in order to better study the mechanism of Weichangshu. MethodsCulture SD rat gastric smooth muscle cells,the cells will be divided into control group of traditional method,cisapride group of traditional method,Weichangshu group of traditional method and control group of improved method,cisapride group of improved method,Weichangshu group of improved method,the 6 groups were treated with different drugs in different ways,then compare growth of cells in each group. ResultsImmunofluorescence of the cultured cells showed that the specific smooth muscle actin was positive.24 h after the third-generation cells were treated with drugs,the cell density in control groups of two methods was not significantly different,cisapride group and Weichangshu group of traditional method were higher than improved method. ConclusionThe improved method can effectively construct drug model of cultured rat gastric smooth muscle cells and get more high purity gastric smooth muscle cells.