参苓白术复方提取物抗放射性肠损伤作用的实验研究

Chinese prescription Shenlingbaizhu Extract prevents radiation-induced small intestinal injury in mice

目的探讨中药参苓白术复方对放射性小鼠肠损伤模型的治疗作用。方法 MTT法检测复方对辐照后小肠上皮细胞的增殖作用。小鼠腹部局部照射后立即灌胃中药复方,测定小鼠体质量,记录腹泻和存活情况,取小鼠空肠切片进行HE染色,观察肠道病理改变。结果体外实验显示,参苓白术复方能明显促进辐照后小肠上皮细胞的增殖。治疗组小鼠一般状况和小肠病理切片明显得到改善,腹泻减少,小鼠存活率明显提高。结论中药复方参苓白术对辐射性肠损伤小鼠有明显的保护作用,为其应用于临床放射性肠损伤提供了实验依据。

ObjectiveTo investigate the therapeutic effect of traditional Chinese prescription Shenlingbaizhu Extract on radiation-induced intestinal injury in mice.MethodsProliferation improvement of irradiated intestinal epithelial cells（IEC-6） was tested by MTT assay in vitro.The preventive effect of the prescription was also tested in vivo.Mice were treated with Shenlingbaizhu by intragastric administration immediately after receiving local irradiation to the abdomen at a dose of 10 Gy（60Co γ-ray）.The body mass,diarrhea and survival were recorded.The pathological changes in the jejunum of mice were stained by HE and observed.ResultsShenlingbaizhu Extract could significantly promote the proliferation of irradiated intestinal epithelial cells in vitro.Shenlingbaizhu Extract treatment reduced the diarrhea of irradiated mice,improved the intestinal structural recovery and increased the mice survival.ConclusionTraditional Chinese prescription Shenlingbaizhu Extract shows significant protective effect on radiation-induced intestinal injury in mice,providing data for clinical treatment of radiation-induced intestinal injury.