磨损颗粒体内诱导血管内皮生长因子的表达

Expression of VEGF induced by wear particle in vivo

[目的]研究植骨气囊模型体内血管内皮生长因子(VEGF)的表达及变化规律,探讨其在人工关节无菌性松动发生过程中的作用。[方法]建立人工关节无菌性松动小鼠植骨气囊模型,在实验组小鼠气囊内注入磨损颗粒悬浮液,对照组气囊内注入生理盐水,分别于植骨后1、2、3周分批处死小鼠,取囊壁连同颅骨片组织行HE染色观察囊壁炎症反应、骨片边缘溶解情况,半定量RT-PCR及免疫组织化学染色法观察VEGF基因及蛋白表达量的变化。[结果]接受磨损颗粒的实验组在各时间点VEGF基因表达量及阳性细胞率均明显高于对照组(P<0.05),其中以2周时表达量最高(P<0.05);且实验组炎症反应、骨片边缘溶解情况及微血管数量也相应地明显于对照组。[结论]磨损颗粒在体内可以诱导VEGF的表达,VEGF在假体周围微血管的生成、炎性细胞浸润及骨溶解中起重要作用。

[Objective]To examine the expression and the change of vascular endothelial growth factor（VEGF）in bone implant in air pouch model,and investigate its significance in the process of aseptic loosening of artificial joints. [Methods]A mouse bone implant in air pouch model for aseptic loosening of artificial joints were established.In the experimental group mouse air pouch was injected with suspension of wear particles,while in the control group with saline alone.All animals were sacrificed at 1,2,3 weeks after bone implantation and the tissues including the exoskeletal plates were harvested for HE staining to observe inflammatory reaction and bone resorption.RT-PCR and immunohistochemistry staining method were applied to detect the gene and protein expression of VEGF in the pouches. [Results]Compared with the control group,VEGF gene expression and the percentage of VEGF＋cells significantly increased（P0.05） in the experimental group,and reached the highest level at 2 weeks after bone implantation（P0.05）.Correspondingly,inflammatory reaction,bone resorption and the number of miro-vessels were much more obvious compared with the control group. [Conclusion]Wear particles could induce VEGF expression in vivo,and VEGF may play an important role in the vascularization of periprosthetic tissue and the process of periprosthetic osteolysis.