β-榄香烯联合热化疗对肺腺癌患者A549细胞系中蛋白表达的影响

Effects of β-elemene combined with chemotherapy on the protein expressions in lung adenocarcinoma A549 cell line

目的探讨β-榄香烯联合顺铂和热疗对非小细胞肺癌A549细胞系杀伤的可能生物学机制。方法不同浓度榄香烯联合热疗和顺铂处理细胞后，用Western blot检测Stat3、p21Waf1／Cip1、Survivin蛋白表达。结果37IC下随着榄香烯浓度的升高，A549细胞中Stat3、磷酸化Stat3（pStat3）和p21Waf1／Cip1蛋白的表达均升高，且高、低浓度组间差异有统计学意义，Survivin蛋白未见明显变化；联合4μg／ml顺铂后，高浓度榄香烯下p21Waf1／Cip1蛋白、Survivin蛋白以及Stat3和pStat3蛋白的表达全部降低；42℃下，榄香烯60／xg／ml组的Stat3蛋白无明显变化，但是pStat3蛋白、p21Waf1／Cip1蛋白和Survivin蛋白均显著下降；榄香烯15μg／ml联合4pg／ml顺铂组Stat3和pStat3蛋白表达均上升，而Survivin表达降低。结论37C下高浓度榄香烯通过p21Waf1／Cip1蛋白高表达抑制A549细胞的生长；高浓度榄香烯联合顺铂主要通过抑制Stat3和pStat3蛋白的表达及使Survivin蛋白降低，抑制细胞生长；42℃下，高浓度榄香烯可能通过抑制Stat3磷酸化为pStat3的过程降低Survivin蛋白表达，促进细胞凋亡；低浓度榄香烯联合顺铂通过降低Survivin蛋白表达导致协同杀伤效应。

Objective To exp1ore the possible molecular mechanisms of β- elemene combined with eisp1atin and heat therapy for killing A549 cell line. Methods The protein expressions of Stat3, p21 Waf1/Cip1 and Surv/vin were detected with Western blot after treatment with 13 elemene of different concentrations combined cisp1atin and heat therapy. Results The protein expressions of Stat3, and pStat3 and p21Waf1/Cip1 in A549 cell line were enhanced with increasing concentrations, and there was significant difference in the expressions between high and low concentration, but Survivin protein had no change at 37℃. After adding 4 μg/mL cisp1atin, the expressions of p21Waf1/ Cip1, Survivin, Stat3 and pStat3 were reduced at β- elemene of high concenlration. At 42℃, there was no significant difference in expression of Stat3 protein at 60 μg/mL elemene, but the expressions of pStat3, p21Waf1/Cip1 and Survivin proteins had sharp1y declined. When using 15 μg/mL elcmene combined with 4 μg/mL cisp1atin, the protein expressions of Stat3 and pStat3 increased, and Survivin expression decreased. Conclusions At temperature of 37℃, β elemene of high concentration may inhibit growth of A549 cells by higher expression of p21Waf1/Cip1 protein, and mainly by inhibiting expressions of Stat3 and pStat3 and Survivin after combined with eisp1atin. At temperature of 42℃ , β- elemene of high concentration may promote apoptosis possibly through inhibition of Star3 phosphorylation and expression of Survivin protein.β-elemene of low concentration combined with cisp1atin leads to synergy killing effect by reducing expression of Survivin protein.