和厚朴酚联合青蒿素体外抗肿瘤的实验研究

Experimental Studies of Honokiol in Combination with Artemisinin on Anti-Tumor Effect of SPC-A-1 Cells in Vitro

目的研究和厚朴酚、青蒿素及二者联合应用对人肺腺癌SPC-A-1细胞生长的抑制作用。方法 MTT法测定和厚朴酚、青蒿素单独或联合对细胞生长的抑制作用,以IC50评判抗肿瘤的效果,应用金氏公式进行联合用药分析的结果;流式细胞仪检测和厚朴酚、青蒿素单独或联合作用对SPC-A-1细胞周期分布的影响。结果和厚朴酚能显著抑制SPC-A-1细胞的增殖,并呈剂量依赖关系,其半数抑制浓度(IC50)为8.20μg.mL-1;青蒿素对SPC-A-1细胞生长没有明显的抑制作用,半数抑制浓度(IC50)为53.52μg.mL-1;和厚朴酚与青蒿素联合应用对SPC-A-1细胞的增殖抑制作用优于各单药组,Q>1.15。细胞周期分布显示联合用药组将细胞阻滞于G1期,显著高于各单药组。结论和厚朴酚单独能够杀伤SPC-A-1细胞,并呈量-效关系,青蒿素单独作用不杀伤SPC-A-1细胞,两者青蒿素联合应用对SPC-A-1细胞具有协同作用。

OBJECTIVE To detect the growth inhibition effect of SPC-A-1 cells treated with honokiol or/and artemisinin. METHODS Using MTT method to measure the growth inhibition rates of SPC-A-1 cells treated with honokiol or/and artemisinin. Using IC50 value to measure the direct anti-tumor effect and applying the King formula to analyze the combination effect of the drugs. Detecting the cell cycle distribution of SPC-A-lcells treated with honokiol or/and artemisinin by FCM combined with PI stai-ning. RESULTS MTF assay showed that honokiol could significantly inhibit the proliferation of SPC-A-1 cells but artemisinin had slightly inhibit the proliferation of SPC-A-lcells. The anti-proliferative effect of honokiol was in a dosage-dependent manner, the IC50 value of honokiol and artemisinin was 8. 58,53.52 μg·mL^-1 respectively. The growth inhibition rates of honokiol combined with artemsinin were much higher than that of the group treated with honokiol or artemsinin, Q 〉 1.15. FCM combined with pI staining analysis demonstrated that honokiol combined with artemsinin treated SPC-A-1 cells for 24 h could induce the cell cycle arrest at G1 phase. CONCLUSION Honokiol could kill SPC-A-1 cells alone in a dosage-dependent manner, while artemisinin could not kill SPC-A-lcells alone. Honokiol combined with artemisinin could produce synergiste effect on SPC-A-1 cells.