雷公藤多苷纳米乳的制备及体外透皮特性研究

Preparation of Tripterygium Wilfordii Multi-Glucoside Nanoemulsion and Transdermal Characterization in Vitro

目的制备雷公藤多苷纳米乳,并对其含量测定、理化性质和体外透皮特性进行考察。方法以肉豆蔻酸异丙酯(IPM)为油相,卵磷脂/烷基葡萄糖苷(APG)为表面活性剂,1,2-丙二醇为助表面活性剂,在室温下采用磁力搅拌法滴加水到油相中,制备雷公藤多苷纳米乳。采用HPLC测定雷公藤多苷纳米乳中雷公藤内酯甲的含量。采用透射电镜和激光粒径测定仪分别测定纳米乳的外观形态和粒径分布。采用Franz扩散池对纳米乳的体外透皮特性进行了考察。结果制备的雷公藤多苷纳米乳为O/W型乳剂,外观圆整、均匀,粒径为31.6 nm,含量稳定。雷公藤多苷纳米乳的稳态透皮速率为14.454 ng.cm-2.h-1,12 h的累积渗透量为166.267 ng.cm-2。结论雷公藤多苷纳米乳可为雷公藤经皮给药提供一种新的剂型。

OBJECTIVE To prepare tripterygium wilfordii multi-glucoside nanoemulsion and investigate its content, physical and chemical properties. Its transderInal properties in vitro were studied as well. METHODS IPM was used as oil phase, lecithin/APG as surfaetant, and 1,2-propanediol as cosurfactant. Water was added dropwisely to the oil phase using magnetic stirring at room temperature. HPLC method was used to determine the content of tripterygium wilfordii lactone armor in the nanoemulsion. Transmission electron microscopy and laser particle size analyzer were used to determine the shape and size of the nanoemulsion. Franz diffusion cell was used for the nanoemulsion transdermal characteristics in vitro. RESULTS The Tripterygium wilfordii muhi-glucoside nanoemulsion was O/W nanoemulsion. Its uniform particle size is 31.6 nm with round appearance and stable content. The steady-state permeation rate was 14. 454 ng·cm^-2·h^-1 and 12 h cumulative amount of penetration was 166. 267 ng·cm^-2. CONCLUSION Tfipterygium wilfordii multi-glucoside nanoemulsion has good transdermal characteristics in vitro. Which is a new dosage form for transdermal drug delivery.