TDGF-1在胃癌中表达的意义及其在上皮间质转化中的作用

The Significance of TDGF-1 Expression in Gastric Cancer and its Role in Epithelial-mesenchymal Transition

探讨胚胎瘤衍生生长因子(teratocarcinoma-derived growth factor-1,TDGF-1)在胃癌中表达的临床意义及其与上皮间质转化(epithelial-mesenchymal transition,EMT)的关系.分别采用免疫组化SP法和逆转录-聚合酶连反应(RT-PCR)方法,检测胃癌组织和正常胃组织中TDGF-1、E-cadherin、Vimentin的表达情况(IHC70例;RT-PCR40例),分析TDGF-1表达与临床病理特征的关系及TDGF-1、E-cadherin、Vimentin三者表达的相关性.TDGF-1、E-cadherin、Vimentin蛋白在胃癌组织中的阳性表达率分别为70%、34.3%、52.9%,在正常胃组织中的阳性表达率分别为38.5%、94.3%、15.7%(P<0.05);TDGF-1、E-cadherin、Vimentin mRNA在胃癌组织中的阳性表达率分别为67.5%、37.5%、52.5%,在正常胃组织中的阳性表达率分别为35%、87.5%、22.5%(P<0.05);TDGF-1蛋白和mRNA的表达均与胃癌的浸润深度、淋巴结转移及TNM分期显著相关(P<0.05);TDGF-1高表达与E-cadherin表达减低显著相关(r=-0.447、P<0.05),TDGF-1高表达与Vimentin表达升高显著相关(r=0.318、P<0.05),E-cadherin表达减低与Vimentin表达升高显著相关(r=-0.283、P<0.05).TDGF-1可能通过调控E-cadherin、Vi-mentin表达促进EMT,从而在胃癌的侵袭转移中发挥重要作用.

To investigate the clinical significance of TDGF-1 expression and the correlation between TDGF-1 expression and epithelial-mesenchymal transition （EMT） in gastric cancer. Immunohistochemical SP and RTPCR methods were used to examine the expression of TDGF-1, E-cadherin, Vimentin proteins and mRNA in gastric cancer and normal gastric tissues （IHC-70 cases; RT-PCR-40 cases）. Then the association between TDGF-1 expression and clinical pathological characteristics as well as the correlation between expression of TDGF-1, E-cadherin, Vimentin were analyzed. The positive expression rates of TDGF-1, E-cadherin, Vimentin protein in gastric cancer were 70%, 34.3% and 52.9% respectively, but the positive expression rates of them in normal gastric tissues were 38.5%, 94.3% and 15.7%（P〈0.05） respectively. The positive expression rates of TDGF-1, E-cadherin, Vimentin mRNA in gastric cancer were 67.5%, 37.5% and 52.5% respectively, but the positive expression rates of them in normal gastric tissues were 35%, 87.5% and 22.5% （P〈0.05） respectively. The expression of TDGF-1 protein and mRNA were significantly associated with depth of invasion, lymph node metastasis and TNM stages （P〈0.05）. The expression of TDGF-1 was closely correlated with that of E-eadherin （r=-0.447, P〈0.05）; Moreover, the expression of TDGF-1 was closely correlated with that of Vimentin （r=0.318, P〈0.05）. On the other hand, the expression of E-cadherin was closely correlated with that of Vimentin （r=-0.283,P〈0.05）. TDGF-1 may promote EMT through the regulation of the e pression of E-cadherin and Vimentin, thereby clearly showing that it plays an important role in invasion and metastasis of gastric cancer.