TMPRSS4在胰腺癌组织中的表达及其临床意义

Expression of TMPRSS4 in pancreatic cancer and clinical significance

目的检测Ⅱ型跨膜丝氨酸蛋白酶4（TMPRSS4）在胰腺癌组织中的表达及其与临床病理特征的关系。方法采用实时PCR法和蛋白质印迹法检测16例胰腺癌和配对癌旁组织TMPRSS4mRNA和蛋白表达；采用免疫组织化学法检测61例胰腺癌标本、26例配对癌旁组织、4例正常胰腺组织TMPRSS4蛋白的表达，分析其与临床病理特征的相关性。结果胰腺癌组织TMPRSS4mRNA和蛋白表达明显高于配对癌旁组织（9．09±7．01比1.27±0．72；1．223±0.125比0．667±0．106，P值均〈0．01），胰腺癌组织TMPRSS4蛋白阳性表达率为67．2％（41／61），显著高于癌旁组织3．8％（1／26）的阳性表达率（P〈0．01）。正常胰腺组织未见TMPRSS4蛋白表达。胰腺癌TMPRSS4表达与患者年龄、性别及肿瘤部位、肿瘤大小无关，而与肿瘤分化程度、淋巴结转移、临床分期密切相关（P值均〈0．05）。结论胰腺癌组织高表达TMPRSS4，其表达与肿瘤的恶性程度相关。

Objective To investigate the expression of TMPRSS4 mRNA, protein in human pancreatic cancer tissues and to explore the relationship between the expression of TMPRSS4 protein and the clinicopathologic parameters. Methods Real-time PCR and Western blotting were used to detect the expressions of TMPRSS4 mRNA and protein in 16 samples of pancreatic cancer tissues and adjacent normal pancreatic tissues. The expression of TMPRSS4 protein in 61 samples of pancreatic cancer tissues and 26 samples of adjacent pancreatic tissues and 4 samples of normal pancreatic tissues was detected by using immunohistochemistry and its relationship with clinicopathological features was analyzed. Results The expression of TMPRSS4 mRNA and protein of pancreatic cancer tissues were significantly higher than those in adjacent pancreatic tissues （9.09 ±7.01 vs. 1.27 ±0.72; 1.223 ±0. 125 vs. 0.667 ±0. 106, P 〈0.01） ; the expression rate of TMPRSS4 protein of pancreatic cancer tissues was 67.2% （41/61）, which were significantly higher than that in adjacent pancreatic tissues [ 3.8% （ 1/26）, P 〈0. 01 ）. There was no TMPRSS4 protein expression in normal pancreatic tissues. There was no significant correlation between the expression of TMPRSS4 protein and the age, gender, tumor location or tumor size was found. There was significant correlation between the expression of TMPRSS4 protein and the degree of differentiation, lymph node metastasis, and clinical staging （P 〈 0.05 ）. Conclusions TMPRSS4 protein is highly expressed in pancreatic cancer tissues, and the expression of TMPRSS4 is associated with the degree of malignancy of pancreatic cancer.