摘要
目的:研究新型复合支架聚乳酸-聚乙醇酸/聚己内酯/纳米羟基磷灰石(PLGA/PCL/nHA)的生物相容性,探讨其作为细胞培养材料和骨组织工程支架的可行性。方法:将兔骨髓基质细胞(bone marrow stromal cells,BMSCs)接种于PLGA/PCL/nHA复合支架上,体外共同培养后,MTT法检测BMSCs增殖活性,电镜下观察PLGA/PCL/nHA复合支架材料表面和孔隙内细胞附着情况。结果:BMSCs在PLGA/PCL/nHA复合支架材料上生长良好。PLGA/PCL/nHA材料表面和孔隙内均有细胞附着,细胞已伸展成梭形或多角形,伸出伪足黏附于材料上。结论:新型PLGA/PCL/nHA复合支架材料生物相容性好,是一种良好的组织工程支架材料,可作为种子细胞的载体应用于骨组织工程中。
Objective： Myriad scaffolding materials and compositions have been and are still being tested for the regeneration of each very specific hard or soft tissue.The objective of this study was to examine a novel composite poly（lactic-co-glycolic acid） / poly（e—caprolactone） / nano-carbonate hydroxyapatite（PLGA/PCL/nHA） on the initial behavior of bone marrow stromal cells（BMSCs） and evaluate the proliferation and differentiation of BMSCs grown on composite scaffold in vitro.Methods： BMSCs cells were cultured on PLGA/PCL/nHA in vitro,cell viability was assessed by MTT reduction assay.Cell adhesion on PLGA/PCL/nHA was observed by scanning electron microscopy（SEM）.Results： BMSCs cells viability were not affected,the proliferation and growth were not affected by PLGA/PCL/nHA,and BMSCs cells could be attached to with a spindle shape or a polygonal shape and extended the pseudopod on PLGA/PCL/nHA.Conclusions： The composite scaffolds is biocompatible and crucial in regulating cellular functions,including survival,adhesion,proliferation,and the like.
出处
《口腔颌面外科杂志》
CAS
2011年第5期326-329,333,共5页
Journal of Oral and Maxillofacial Surgery
基金
上海市科学技术委员会资助项目(0852nm03600)
