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Caspase-3体外对PIAS1蛋白酶切作用的初步验证 被引量：1

Cleavage effect of Caspase-3 on PIAS1 protein in vitro
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摘要 目的观察凋亡相关蛋白Caspase-3体外对PIAS1蛋白的酶切作用,为后续研究奠定基础。方法以HEK293T细胞表达PIAS1蛋白,采用免疫共沉淀(Co-IP)实验观察其与Caspase-3间的结合作用;采用体外蛋白酶切实验检测Caspase-3对PIAS1的酶切作用。结果 Co-IP实验发现体外Caspase-3可与PIAS1蛋白结合;蛋白酶切实验发现PIAS1的酶切片段。结论 Caspase-3与PIAS1在体外具有相互作用,PIAS1体外可被Caspase-3酶切,为后续研究奠定了基础。 Objective To observe the cleavage effect of Caspase-3on PIAS1protein in vitro.Methods PIAS1protein was expressed in HEK293Tcells.Co-immunoprecipitation（Co-IP）was used to observe the binding between PIAS1protein and Caspase-3.The cleavage effect of Caspase-3on PIAS-1protein was detected by in vitro proteolytic cleavage test.Results Co-IP result showed that Caspase-3bond to PIAS1 in vitro.Proteolytic cleavage test showed that PIAS1was cleaved by active Caspase-3.Conclusion It has been shown that Caspase-3can interact with PIAS1protein in vitro,and PIAS1can be cleaved by Caspase-3 in vitro.
作者 赵瑞 唐春花 赵文元 刘建民 洪波 许奕 黄清海 霍克克
机构地区 第二军医大学长海医院临床神经医学中心 复旦大学生命科学学院遗传学研究所
出处 《第二军医大学学报》 CAS CSCD 北大核心 2011年第10期1144-1146,共3页 Academic Journal of Second Military Medical University
基金 国家自然科学基金(30872663)~~
关键词 CASPASE-3 PIAS1 酶切 体外 Caspase-3 PIAS1 cleavage in vitro
分类号 R349.5 [医药卫生—基础医学]
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参考文献13

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共引文献1

同被引文献20

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第二军医大学学报
2011年 第10期

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