钩藤中钩藤碱的提取和含量测定

Extraction and Content Determination of Rhynchophylline from UNCARIAE RAMULUS CUM UNCIS

[目的]研究钩藤中钩藤碱的提取和含量测定方法。[方法]采用生物碱回流提取方法提取,采用高效液相色谱法对钩藤中钩藤碱的含量进行测定并计算提取率。[结果]专属性考察结果显示,钩藤碱的保留时间约为18.843 min,异钩藤碱的保留时间约在22.323min,两峰分离度良好,且钩藤药材中其他成分不干扰测定;含量测定结果显示,钩藤碱和异钩藤碱的含量分别为23.23、129.17μg/g,加样回收试验平均回收率分别为99.12%、101.33%,RSD分别为1.84%、1.56%。[结论]该方法稳定、有效、准确度高,可用于钩藤中钩藤碱的含量测定。

[Objective] The aim was to study on the extraction and content deter mination of rhynchophylline from UNCARIAE RAMULUS CUM UNCIS.[Method] Extraction method of traditional alkaloids was adopted.And modern analysis method HPLC was used to deter mine the content of rhynchophylline.Rhynchophylline content in UNCARIAE RAMULUS CUM UNCIS was calculated.[Result] Specialization investigation showed that the retention time of rhynchophylline was about 18.843 min,and that of isorhynchophylline was 22.323 min.The two peaks showed good resolution;and other components in UNCARIAE RAMULUS CUM UNCIS did not interfere with the assay.Results of content deter mination showed that the contents of rhynchophylline and isorhynchophylline were 23.23 and 129.17 μg/g,respectively.The average recovery rates of added recovery test were 99.12% and 101.33%,with RSD values being 1.84% and 1.56%,respectively.[Conclusion] This method was stable,effective,accurate,and could be used for the content deter mination of rhynchophylline in UNCARIAE RAMULUS CUM UNCIS.