产碱性纤维素酶菌株的筛选及酶学性质研究

Screening of Alkaline-Cellulose Producing Strain and Study on Its Enzymaic Characterization

从采集的造纸厂土样中,筛选出l株产碱性纤维素酶酶活力较高的菌株H-1,经鉴定,该菌株为革兰氏阳性芽孢杆菌属。发酵产酶条件和酶学性质研究表明H-1菌株的适宜发酵条件为:接种量为6%,pH 9.0,温度为37℃,此条件下的酶活力可达8.69 U/mL,是初始酶活力(2.93 U/mL)的3倍。该酶最适反应温度为40℃,最适反应pH 9.0。在25～55℃,pH 7.0～11.0仍能保持60%以上的酶活力,能较好地满足日常洗涤的环境要求。

From soil samples collected from paper mills,one strain called H-1 capable of degrading cellulose was screened and identified as Gram-positive Bacillus.The optimum enzyme-producing conditions were as follows,inoculum amount 6%,pH 9.0,temperature 37 ℃.The enzyme activity was up to 8.69 U/mL,which was 3 times higher than the initial enzyme activity（2.93 U/mL）.The optimal conditions for the enzymatic reaction were about 40 ℃ and pH 9.0.The enzyme activity could be maintained above 60% at temperature 25～55℃ and pH 7.0～11.0,which meet the environmental requirements of the daily washing