生长抑制基因1在喉鳞状细胞癌中的突变检测及与p53蛋白表达关系的研究

Mutation of ING1 gene in laryngeal squamous cell carcinoma and its association with p33ING1b protein expression

目的:研究喉鳞状细胞癌组织中生长抑制基因1(ING1)基因的突变情况,并探讨p33ING1b与p53蛋白表达的关系。方法:抽提33例石蜡固定喉鳞状细胞癌组织DNA,PCR扩增ING1第2外显子序列,测序检测突变情况;免疫组织化学法检测喉鳞状细胞癌p33ING1b蛋白与p53蛋白的表达水平,并分析两者之间的关系。结果:喉鳞状细胞癌组织中ING1基因无突变现象,但2例组织中出现编码区第170个密码子核苷酸多态性位点,由GGG变为AGG;免疫组织化学检测结果显示p33ING1b、p53蛋白表达与喉鳞状细胞癌临床病理参数之间无相关性。但配对χ2分析显示p33ING1b、p53蛋白表达有明显差异,在p53阳性的病例中,p33倾向于阴性表达。结论:喉鳞状细胞癌中ING1基因的突变较少见,在正常生理环境中,p33ING1b可能与野生型p53蛋白协同发生作用。

Objective：To investigate the ING1 gene mutation status in human laryngeal squamous cell carcinoma（LSCC）,and the association of p33ING1b protein expression with p53 protein expression.Method：DNA of LSCC tissue was extracted,and nucleotide of the second exon was amplified and sequenced to determine the chromosome status.The p33ING1b and p53 protein expression were detected by immunohistochemistry and the association between them were analyzed.Result：No mutation was detected in ING1 gene,but a single polymorphism from GGG to AGG at codon 170 of ING1 gene was found in 2 of the 25 LSCC tissues,The immunohistochemical analysis showed that 4 had positive p33ING1b expression.No association was found between p33ING1b expression and LSCC clinical features,or between p53 and clinical features.However,significant difference was found between p33ING1b and p53 expression.p33ING1b tended to be negative in p53 expression positive tissue.Conclusion：ING1gene mutation appears rare in LSCC.In normal physical condition,p33ING1b may play a synergistic effect with p53 protein.