仙人掌总黄酮抗炎作用的研究

Study on the anti-inflammatory effect of total flavonoids from Opuntia monacantha(Willd.) Haw

为考察两种不同工艺(水提醇沉,超声波醇提)提取的仙人掌总黄酮的抗炎作用,通过冰醋酸致小白鼠腹腔毛细血管通透性增加,棉球致小白鼠肉芽肿以及蛋清致大白鼠足跖肿胀的炎症模型,观察两种仙人掌总黄酮对不同炎症的作用效果。结果显示,水提醇沉高剂量组和超声波醇提高剂量组的仙人掌总黄酮均能极显著抑制冰醋酸所致的小白鼠腹腔毛细血管通透性增加(P<0.01),且高剂量水提醇沉总黄酮的作用效果优于阳性药物地塞米松磷酸钠的作用效果;水提醇沉和超声波醇提总黄酮的各个剂量都能极显著抑制棉球所致小白鼠肉芽肿(P<0.01),其中水提醇沉高剂量和超声波醇提低剂量的作用效果与地塞米松磷酸钠的作用效果相近,但超声波醇提总黄酮毒性过大,不适于临床运用。水提醇沉总黄酮的各剂量均能不同程度地抑制蛋清引起的大白鼠足趾肿胀,高剂量可极显著降低肿胀率(P<0.01),作用效果与地塞米松磷酸钠接近。试验结果证明,仙人掌总黄酮对于急、慢性炎症均具有良好的抑制作用。

To investigate the anti-inflammatory effect of total flavonoids from Opuntia monacantha（Willd.） Haw,total flavonoids was extracted with two different methods including water extraction alcohol precipitation method（WEAP） and ultrasonic wave-alcohol extraction method（UWAE）.The anti-inflammatory effect was investigated through three models including acetic acid-induced peritoneal capillary permeability in mice,cotton-pellet granuloma of the mice and egg white-induced rat paw oedema.In result high-dosage of total flavonoids prepared from WEAP and UWAE methods had both significant inhibitory effects on penetration of capillary vessel in mice（P0.01）.Moreover,compared with dexamethasone so-dium phosphate,high-dosage of WEAP had a better anti-inflammatory effect.Different doses of total flavonoids prepared from WEAP and UWAE methods also significantly decreased the proliferation of granuloma caused by implantation cotton pellet in mice（P0.01）,and anti-inflammatory effect of the high-dosage of WEAP and low-dosage of UWAE was similar to dexamethasone sodium phosphate.Nonetheless,total flavonoids prepared by UWAE method was associated with excessive toxicity and was not suitable for me-dical use.High-dosage of total flavonoids prepared with WEAP method exhibited good attenuation effects on rat paw edema induced by egg white（P0.01）,with its anti-inflammatory effect similar to dexamethasone sodium phosphate.In conclusion,total flavonoids from Opuntia monacantha（Willd.） Haw has remarkable anti-inflammatory effects in the animal models of both acute and chronic inflammation.