槲皮素对乳腺癌MCF-7细胞热休克蛋白表达的影响

Effects of quercetin on heat shock protein expression in human breast cancer cell MCF-7

目的:探讨槲皮素对乳腺癌MCF-7细胞株热休克蛋白70(HSP70)、HSP27和HSP90的表达的影响。方法:对体外培养的乳腺癌MCF-7细胞,恒温42℃热处理2h,分别于4、8、12和24h后荧光定量PCR检测HSP70、HSP27和HSP90的基因表达变化并用蛋白质印迹检测蛋白表达量的变化。观察热处理前使用150μmol/L槲皮素对乳腺癌MCF-7细胞HSP70、HSP27和HSP90的基因表达变化、蛋白表达量的影响。结果:42℃热处理后乳腺癌MCF-7细胞HSP70、HSP27和HSP90表达在基因及蛋白水平上均有明显升高,4h达到高峰,8h后开始减少,24h接近正常值。槲皮素能抑制HSP70、HSP27表达,但导致HSP90表达水平先升高,4～8h表达仍处于高峰值,8h后才开始下降。结论:HSP70、HSP27和HSP90在MCF-7细胞内的基因和蛋白表达均在热处理后迅速升高,在热处理前使用槲皮素可以抑制HSP70、HSP27的表达,HSP90水平升高及下降延迟。

OBJECTIVE： To investigate the effects of quercetin on heat shock protein expression in human breast cancer cell MCF-7.METHODS： MCF-7 cells were incubated at 42 ℃ for 2 h in vitro.The changes of gene expression and protein levels of HSP70,HSP27 and HSP90 were detected by real-time PCR and Western-blot after 4,8,12 and 24 hours.MCF-7 cells were pretreated with 150 μmol/L Quercetin before heat treatment,then detected the changes of gene expression and protein levels of HSP70,HSP27 and HSP90.RESULTS： The gene expression and protein levels of HSP70,HSP27 and HSP90 reached to its peak at 4 h,decresed at 8 h and returned to normal level at 24 h after heat treatment.Quercetin could inhibit gene expression and protein levels of HSP70 and HSP27,but up-regulated and delayed descent of HSP90.The level stay in peak at 4 to 8 h and then decrease to normal.CONCLUSIONS： The gene expression and protein levels of HSP70,HSP27 and HSP90 up-regulate quickly after heat treatment.Using quercetin before heat treatment can inhibit gene expression and protein levels of HSP70 and HSP27,but increase and delay that of HSP90.