丝胶对2型糖尿病大鼠睾丸c-fos表达的影响

Effects of sericine on c-fos expression in testis of type 2 diabetic mellitus rats

目的观察2型糖尿病(T2DM)大鼠睾丸原癌基因c-fos表达的变化及丝胶的保护作用。方法 30只雄性SD大鼠随机分成正常对照组、糖尿病模型组和丝胶治疗组。链脲佐菌素腹腔注射建立T2DM大鼠模型,以血糖≥16.7 mmol/L作为成模标准。待模型成功建立后,模型组大鼠不做任何处理;丝胶治疗组大鼠给予丝胶灌胃(2.4 g·kg-1·d-1)35 d。ELISA方法检测大鼠血清睾酮水平,SP免疫组化法和RT-PCR法分别检测睾丸c-fos蛋白和mRNA的表达。结果 T2DM模型大鼠血清睾酮水平、睾丸c-fos蛋白和mRNA的表达均明显低于正常对照组大鼠(P<0.01);丝胶治疗组大鼠血清睾酮水平、睾丸c-fos蛋白和mRNA的表达明显高于模型大鼠(P<0.01,P<0.05)。结论丝胶可通过上调睾丸c-fos的表达,促进精原细胞向精母细胞转化,发挥对T2DM生殖功能损伤的保护作用。

Objective To observe the changes of c-fos expression in testis of type 2 diabetic mellitus（T2DM） rats and the protective effects of sericine.Methods 30 male SD rats were randomly divided into normal control,model and sericine treatment groups（n=10）.T2DM model rats were made by continuous intraperitoneal injection of streptozotocin and blood glucose≥16.7 mmol/L was taken as standard.After successfully establishing model,the rats in model group were not given any more treatment;the rats in sericine treatment group were lavaged with sericine（2.4 g·kg-1·d-1） for 35 days.ELISA was used to detect the testosterone level in serum;SP immunohistochemical staining and RT-PCR were used to detect the expression of c-fos protein and mRNA in testis respectively.Results The testosterone level in serum,the c-fos protein and mRNA expression in testis of diabetes model rats were obviously lower than those of normal control rats（P0.01）.The testosterone level in serum,the c-fos protein and mRNA expression in testis of rats in sericine treatment group were significantly higher than those of diabetes model rats（P0.01,P0.05）.Conclusions Sericine can protect the spermatogenic function of T2DM rats by up-regulating the c-fos expression in testis and promote transformation from spermatogonia to spermatocyte.