ACE、AGT基因联合多态性与高血压合并脑梗塞相关

Association between the gene polymorphism of ACE and AGT in hypertension disease complicated with cerebral infraction

目的探讨北京地区汉族人群血管紧张素转化酶(ACE)基因插入/缺失(I/D)多态性与血管紧张素原(AGT)基因CD235Met-Thr变异(M235T)与高血压合并脑梗塞发生的关系。方法分别用PCR法、突变基因分离聚合酶链反应(MS-PCR)法检测664例高血压合并脑梗塞患者(CI),678例单纯高血压患者(EH)和716例对照者(C)的ACE基因I/D多态性及AGT基因M235T多态性,分析两个基因多态性分布与高血压合并脑梗塞发病的相关性。结果 CI组ACE-DD和AGT-TT基因型频率(分别为0.309和0.643)均显著高于C组(分别为0.203和0.543,P<0.001)和EH组(分别为0.217和0.569,P<0.01)。CI组与C组比较,ACE-DD、AGT-TT联合基因型OR值(2.547,95%CI:1.919～3.382)明显高于ACE-DD单基因型(1.759,95%CI:1.376～2.248)和AGT-TT单基因型(1.515,95%CI:1.220～1.880)。结论 ACE-DD和AGT-TT基因型与北京地区汉族人群单纯高血压发病无相关性,但与高血压合并脑梗塞发病显著相关,并且ACE-DD和AGT-TT联合基因型是高血压合并脑梗塞发病的危险因素。

Objective To explore the association between the ACE,AGT gene polymorphism and hypertension disease complicated with cerebral infraction.Methods ACE gene I/D polymorphism and the AGT gene M235T polymorphism were detected in 664 cerebral infraction（CI） cases,678 simple essential hypertension（EH） cases and 716 control cases（C）.The association of gene polymorphism and hypertension disease complicated with cerebral infraction was evaluated.Results The genotype frequencies of ACE-DD and AGT-TT in the CI group（0.309 and 0.643） were significantly higher than those in the control group（0.203 and 0.543） and EH group（0.217 and 0.569）,respectively.The OR value of combining ACE-DD and AGT-TT of CI to the control group（2.547,95% CI：1.919-3.382） was significantly higher than the OR value of single ACE-DD genetype（1.759,95% CI： 1.376-2.248） or single AGT-TT genetype（1.515,95% CI：1.220-1.880）.ConclusionThese results suggest that ACE-DD or AGT-TT variant is not associated with Chinese hypertensive patients,but is potentially associatedwith Chinese hypertensive patients complicated with cerebral infraction.ACE-DD and AGT-TT may have a synergistic effect to risk of hypertension disease complicated with cerebral infraction.