腺病毒介导的人视网膜母细胞瘤94基因对人食管癌细胞辐射增敏作用的研究

Radiosensitivity enhancement of recombimant adenoviral-mediated retinoblastoma 94 gene in human esophageal carcinoma cells

目的探讨重组腺病毒人视网膜母细胞瘤94基因（Ad—Rb94）对吖射线杀伤人食管癌EC109细胞的增敏作用。方法将Ad—Rb94体外转染后的EC109细胞，按数字随机表法，分为空白对照组、Ad-LacZ对照组、Ad-Rb94组、照射组和Ad—Rb94联合照射组，观察EC109的细胞的抑制率、细胞周期和Rb蛋白的表达。结果Ad-Rb94组、照射组和Ad—Rb94联合照射组对EC109细胞生长均具有抑制作用，Ad—Rb94联合照射组的抑制效应最强，明显高于Ad—Rb94组和照射组（F=23．31，P〈0．05）。Ad—Rb94联合照射组EC109细胞出现明显的G2期阻滞，G2期细胞所占比例达50％。Ad-Rb94联合照射组表达Rb蛋白的细胞明显增加，阳性率达71％，较Ad—Rb94组和照射组差异有统计学意义（x^2=8．31和6．73，P〈0．05）。结论Rb9d基因联合照射能明显提高对人食管癌细胞的辐射增敏性。

Objective To study the radiosensitivity enhancement effects of recombinant adenovirus mediated retinoblastoma 94 gene on the growth of esophageal carcinoma cells EC109. Methods EC109 cells was transfected with recombinant Rbg4 gene adenovirus and irradiated by ^137Cs γ-rays. The cohorts were divided into groups as blank control, Ad-LacZ, Ad-Rb94, radiation and Ad-Rb94 combined with radiation. Cell inhibition ratio, cell cycle and expression of retinoblastoma protein of EC109 cells were analyzed. Results The growth of EC109 cells transfected with Ad-Rb94, radiation and Ad-Rb94 combined with radiation group was all inhibited. The group of Ad-Rb94 combined with radiation resulted in greater inhibition of cells growth compared with Ad-Rb94 group and radiation group （F = 23.31, P 〈 0.05）. Cells of G2 phases of EC109 cancinoma cells for Ad-Rb94 combined with radiation group were the highest, which was 50%. The combination of Ad-Rb94 and radiation group resulted in the greatest expression of retinoblastoma protein, which reached 71% , significantly higher than Ad-Rb94 infection and radiation groups （ x^2 = 8.31,6.73, P 〈 0. 05 ）. Conclusions Retinoblastoma 94 gene combined with ionizing radiation can enbance the radiation sensitivity of EC109 cells,