摘要
目的:研究维拉帕米对人肝星状细胞(Hepatic stellate cells,HSCs)凋亡的影响以及凋亡相关蛋白Bcl-2/Bax表达的变化。方法:CCK-8法检测不同浓度的维拉帕米处理48 h对HSCs细胞增殖的抑制作用;流式细胞仪AnnexinV/PI双染法检测HSCs细胞的凋亡率;免疫组化SABC法检测Bcl-2/Bax表达的变化。结果:一定浓度范围的维拉帕米对HSCs细胞的增殖有抑制作用,随浓度增加,抑制作用增强,其IC50值为(0.15±0.01)mol/L;AnnexinV/PI双染法检测显示,不同浓度的维拉帕米均可诱导HSCs细胞凋亡,与阴性对照组比较,差异有统计学意义(P<0.05);免疫组化检测显示,与对照组比较,维拉帕米组Bcl-2表达减少,而Bax表达增强,与阴性对照组比较,差异有统计学意义(P<0.05)。结论:维拉帕米可抑制HSCs细胞的增殖,诱导其凋亡,作用机制可能与抑制Bcl-2、增强Bax的表达有关。
Objective：To study the effects of Verapamil on apoptosis and the expression of Bcl-2/Bax of human hepatic stellate cells（HSCs）.Methods：HSCs were treated with different concentrations of Verapamil for 48 h.The inhibitory rate of Verapamil on HSCs was determined by CCK-8 assay;the rate of apoptosis was detected by flow cytometry with Annexin-V/PI staining;and the expression of Bcl-2/Bax was tested by SABC of immunohistochemical method.Results：Different concentrations of Verapamil had certain inhibitory efffct on HSCs cell proliferation in a dose-dependent manner,and the IC50 value was（0.15±0.01） mol/L.The apoptosis of HSCs was induced by different concentrations of Verapamil,which was statistically different from the negative control group（P〈0.05）.The expression of Bcl-2 of HSCs cells was decreased and the expression of Bax was increased after treatment of Verapamil,which was statistically different from the negative control group（P〈0.05）.Conclusion：Verapamil can inhibit the proliferation and induce the apoptosis of HSCs cells,and its mechanism might be related to decreasing the expression of Bcl-2 and increasing the expression of Bax.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2011年第9期1037-1039,共3页
Journal of Chongqing Medical University
基金
泸州医学院青年基金资助项目(编号:256)
