TGF-β_1和α-SMA在大鼠皮层注射FeCl_3所致痫性发作中的表达

The expression of TGF-β_1 and α-SMA between young and adult rats after intracortical injection of ferric chloride

目的:研究转化生长因子β1(Transforming growth factor beta 1,TGF-β1)和α-平滑肌肌动蛋白(Alpha-smooth muscleaction,α-SMA)的表达差异与幼年和成年大鼠皮层注射FeCl3所致痫性发作的相关性,探讨其在大鼠痫性发作中的可能机制。方法:分别用FeCl3和生理盐水(Normal saline,N.S)注射幼年与成年大鼠皮层,观察大鼠痫性发作情况。ELISA法检测脑脊液(Cerebral spinal fluid,CSF)中TGF-β1变化,RT-PCR和Western免疫印迹法检测注射区域α-SMA表达。结果:只有幼鼠FeCl3组出现癫痫样发作;TGF-β1和α-SMA在幼鼠FeCl3组表达最高。结论:幼年比成年鼠更易并发癫痫发作,且TGF-β1和α-SMA表达更高,推断TGF-β1和α-SMA表达增加是幼鼠更易并发痫性发作的重要机制之一。

Objective：To study the correlation between expressions of TGF-β1 α-SMA and seizures of rats after intracortical injection of ferric chloride and explore its role in occurrence of rats seizure.Methods：Rats（childhood and adulthood） were treated with an intracortical injection of FeCl3 and normal saline,the occurrence of seizures were under observation;TGF-β1 in cerebral spinal fluid（CSF）was tested by ELISA;the expressions of mRNA and protein of α-SMA were detected by RT-PCR and Western blotting.Results：Epilepsy seizure was only observed in FeCl3 childhood group;TGF-β1 and α-SMA expression ranks top 1 in FeCl3 childhood group.Conclusion：Childhood rats were more susceptible to seizure than adulthood rats with higher level of TGF-β1 and α-SMA expressions;implying that the growth of TGF-β1 and α-SMA expressions might be the one of key mechanisms in seizure.