老年男性外周血CD4^+和CD8^+ T细胞中CD45RA^+和HLA-DR^+的表达研究

Expression of CD45RA^+ and HLA-DR^+ on peripheral blood CD4^+ and CD8^+ T lymphocyte in aged males

目的探讨不同年龄组老年男性外周血CD4+和CD8+T细胞中CD45RA+和HLA-DR+的表达。方法采用逐步入组方法入组101例老年男性,分青老年组(65岁及以上),中老年组(75岁及以上)和老老年组(85岁及以上)三组,用多色流式细胞仪检测外周血CD45RA+和HLA-DR+的表达,随访两年。结果各组间CD45RA+和HLA-DR+T细胞的表达轻微下降,其中仅老老年组与青老年组之间CD4+HIL-DR-和CD8+HIL-DR+T细胞的表达差异有统计学意义(P<0.05)。结论老年男性外周血CD4+和CD8+T细胞中CD45RA+和HLA-DR+的表达可能存在随龄性下降趋势。

Objective To study the expression of CD45RA＋ and HLA-DR＋ on peripheral blood CD4＋ and CD8＋T lymphocyte of different aged male groups.Methods Using the gradual enrollment method,101 aged males were divided into three groups： young aged group（65 years old and over）,middle aged group（75 years old and over） and old aged group（85 years old and over）.Expression of CD45RA＋ and HLA-DR＋T lymphocyte from peripheral blood were detected with multi-colors flow cytometer,following up two years.Results Expression of CD45RA＋ and HLA-DR＋T lymphocyte in different groups slightly decreased,expression of CD4＋HIL-DR-and CD8＋HIL-DR＋T lymphocyte between old aged group and young aged group had a significant difference（P0.05）.Conclusion Expression of CD45RA＋ and HLA-DR＋ on peripheral blood CD4＋and CD8＋ T lymphocyte of aged male groups has a potential decline trend with increase of age.