骨髓间充质干细胞关节腔注射治疗胶原诱导性关节炎的实验研究

The effect of injection of bone mesenchymal stem. cell into the joints of collagen-induced arthritis rats

目的观察骨髓间充质干细胞（BMSCs）关节腔注射治疗胶原诱导性关节炎（CIA）的疗效。方法体外扩增原代间充质干细胞至P2代。大鼠胶原二次免疫后第7天，将等量0．9％氯化钠注射液及P2代细胞分别注射至CIA不同侧足踝关节处。2，4，8，12周后观测关节炎指数和足爪关节X线分析及指数评估；12周处死动物进行组织学观察。采用配对t检验进行统计学分析。结果在不同观察时间点间充质干细胞治疗组与对照组的关节炎指数为：（第2周）3．18±0．62与3．84±0．35，（第4周）3．45±0．28与4．06±0．38，（第8周）3．86±0．23与6．75±0．36，（第12周）4．23±0．43与7．86±0．66；X线放射学指数为：（第2周）2．04±0．21与2．72±0．15，（第4周）2．52＋0．47与4．06±0．38，（第8周）3．56＋0．29与4．35±0．36，（第12周）3．73±0．43与4．86±0．62；组织学评分为：（第12周）2．34±0．22与3．52±0．55。结论骨髓间充质干细胞关节腔注射治疗可以抑制CIA大鼠的病情发展，有望成为一种新的临床治疗手段。

Objective To evaluate the effect of Bone mesenehymal stern cells （BMSCs） intra- articular injection on the pathological development of collagen-induced arthritis （CIA） rats. Methods CIA Wistar rats were divided into 2 groups： the saline injection control group and the BMSCs injection group. BMSCs were isolated from normal rats and cultured in vitro till P2 generation, which were injected into the affected the ankle joint consequently. After 2, 4, 8, 12 weeks of processing, the following indexes were observed： arthritis score and X-ray. Twelve weeks later, all of the rats were sacrificed and the following histological indexes were evaluated： cartilage destruction and cartilage extracellular matrix secretion. Paired samples t-test was used for the statistical comparison. Results All the arthritis indexes of the BMSCs group were much lower than those of the saline control group. The arthritis scoreS were 3.18±0.62 vs 3.84±0.35 （at week 2）, 3.45±0.28 vs 5.96±0.48 （at week 4）, 3.86±0.23 vs 6.75±0.36 （at week 8）, 4.23±0.43 vs 7.86± 0.66 （at week 12） respectively. X-ray scores were 2.04±0.21 vs 2.72±0.15 （at week 2）, 2.52±0.47 vs 4.06± 0.38 （at week 4）, 3.56±0.29 vs 4.35±0.36 （at week 8）, 3.73±0.43 vs 4.86±0.62（at weekl2） respectively and the histological evaluation indexes were 2.34±0.22 vs 3.52±0.55 （at week 12）. Coneinsion BMSCs injection may inhibit the pathological development of CIA rats, and it may be a promising approach for the treatment of rheumatoid arthritis.