激活血管平滑肌细胞OX40-OX40L系统对其增殖及迁移能力的影响

Effects of OX40-OX40L system activation on proliferation and migration of cultured rat aortic vascular smooth muscle cells

目的研究OX40-OX40L轴的激活对大鼠血管平滑肌细胞(VSMC)增殖和迁移能力的影响。方法原代培养大鼠主动脉VSMC并传代,培养液中添加OX40L(0、0.1、1、10或20mg/L)刺激24h,或在培养基中加入选定浓度OX40L10mg/L,刺激6、12、24、36、48h。以CCK-8法测定VSMC增殖率,以划痕法测定VSMC迁移率。结果在OX40L浓度从0～20mg/L范围内,刺激24h后,VSMC的增殖、迁移能力的增强与OX40L浓度呈剂量依赖性,并明显大于OX40L0mg/L组(P<0.05)。OX40L浓度达到10mg/L时,VSMC的增殖、迁移能力已接近最大值。应用10mg/L的OX40L刺激不同时间(6、12、24、36、48h)后,发现随着刺激时间的延长,VSMC增殖、迁移能力逐渐增强;刺激至24h,VSMC增殖能力达到最大值。结论 OX40-OX40L轴的激活可能通过促进VSMC的增殖及迁移影响动脉粥样斑块的进展。

Objective To investigate the effects of OX40-OX40 ligand activation on the proliferation and migration of primarily cultured aortic vascular smooth muscle cells（VSMCs） in rats.Methods The VSMCs were cultured and passaged.The OX40L in the varied concentrations of 0 to 20 mg/L was added into culture medium and cultured for 24 hours,or OX40L 10 mg/L was added into culture medium and cultured for 6 to 48 hours.The proliferation and migration of VSMCs were detected by CCK-8 kits and wound-healing assay.Results The proliferation and migration of VSMCs were significantly increased by OX40L stimulation in a dose-dependent manner,which reached the maximum when concentration of OX40L was 10 mg/L（P〈0.05）.The maximum rates of proliferation and migration of VSMCs were seen when OX40L 10 mg/L was used to activate OX40-OX40L system for 24 hours（P〈0.05）.Conclusion The activating of OX40-OX40L system promotes the proliferation and migration of VSMCs,which may affect the progress of artherosclerosis plaque.