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穿膜融合多肽TAT-N24对胃癌细胞迁移的影响 被引量：1

Influence of Cell-permeable TAT-N24 Fusion Peptide on Cell Migration of Gastric Carcinoma Cells
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摘要 目的观察穿膜融合多肽TAT-N24对胃癌细胞MGC803迁移的影响。方法用纯化的融合多肽TAT-N24处理胃癌MGC803细胞,通过细胞迁移实验检测其对细胞运动迁移能力的影响,明胶酶谱实验观察其对肿瘤转移相关基因MMP9表达的影响。结果经TAT-N24多肽处理的MGC803细胞,其细胞迁移能力明显低于对照组细胞,差异具有统计学意义(P<0.05),但其对肿瘤转移相关基因MMP9的表达和分泌却没有影响。结论融合多肽TAT-N24能够抑制胃癌MGC803细胞的运动迁移能力,其在胃癌的治疗上可能具有潜在的应用前景。 Objective To observe the effects of cell - permeable TAT - N24 fusion peptide derived from p553, regulatory subunit of pbosphoinositide -3 kinase on cell migration of MGCS03 gastric carcinoma cells. Methods Gastric cancer cells MGC803 was treated with the purified fusion peptide TAT - N24. The ability of cell migration was detected by transwell migration assay. Expression of tumor metastasis associated gene MMP9 was observed by gelatin zymography. Results TAT - N24 fusion protein markedly resulted in decrease of the MGC803 cell migration compared with the control cells （P 〈 0.05 ）. But it did not affect the expression and secretion of MMP9. Conclusion TAT - N24 fusion peptide could inhibit cell migration in gastric carcinoma MGC803 cells. It perhaps has potential clinical application in gastric carcinoma.
作者 刘秀财 郭红艳 高涵 齐晓丹 兴桂华 王淑英 孙晓杰
机构地区 齐齐哈尔医学院生物化学教研室
出处 《医学研究杂志》 2011年第10期69-71,共3页 Journal of Medical Research
基金 黑龙江省教育厅面上项目课题(11531431) 齐齐哈尔市科技局指导项目
关键词 TAT—N24 胃癌 细胞迁移 磷脂酰肌醇3-激酶(PI3K) TAT- N24 Gastric carcinoma Cell migration PI3K
分类号 R977.21 [医药卫生—药品]
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参考文献11

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二级参考文献38

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共引文献10

同被引文献2

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医学研究杂志
2011年 第10期

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