钐对小鼠睾丸组织超微结构的影响

Effects of sub-chronic samarium exposure on ultra-structure of mice testis

为评价稀土元素钐对雄性小鼠睾丸组织结构的影响,将小鼠分组,并饮用含硝酸钐0、5、50、500、2000mg/L的蒸馏水,90d后处死,称量体重和睾丸重量,制备光镜和电镜切片.结果表明,与对照组相比,2000mg/L剂量组的小鼠睾丸脏器系数显著降低;光镜观察显示高剂量组的小鼠睾丸曲细精管萎缩、变性,结构紊乱,生精细胞明显减少;电镜观察显示高剂量的钐可致曲细精管管膜萎缩,各级生精细胞均受到不同程度的损伤,线粒体普遍出现肿胀、空泡化现象,核膜模糊或缺失,染色质边集.提示500,2000mg/L剂量下的钐暴露对小鼠的睾丸组织具有一定的损伤效应.

In order to evaluate the sub-chronic toxicity of rare earth element samarium on testis of male mice, the mice were randomly selected and exposed to drinking water containing 0, 5, 50, 500, 2000mg/L of samarium nitrate for 90d. At the end of treatment, the mice were weighed and euthanized, and the organ coefficients were determined.The histopathology and electron microscopy observations were evaluated as well. Compared with the control group, the organ coefficient of testis from 2000mg/L group was significantly decreased;the seminiferous tubule of testis from high dose showed increased atrophies, vacuolation, and the germ cell count showed an obvious decrease and displayed disorderly organization.Electron microscopy observation showed that tubular membrane with higher dose was impaired, mitochondria were substantially swelled and vacuolated, nucleus membrane showed blur or was missing, and chromatin was marginated. Results indicated that under sub-chronic exposure of samarium with concentrations of 500mg/L and 2000mg/L, samarium exerted some damaging effect on mice testis.