心脑疏通胶囊对动脉粥样硬化家兔IL-10、TNF-α水平的影响

Effects of GSTT on levels of IL-10 and TNF-α in atherosclerotic rabbits

目的:观察蒺藜总皂苷对动脉粥样硬化家兔的血脂、血清IL-10、TNF-α水平的影响,并探讨其对动脉粥样硬化的治疗作用。方法:取50只健康雄性日本大耳白兔,随机取10只作为空白对照组,喂以普通饲料,其余40只给予高脂饮食喂养4周后,再随机分为四组:1模型组,10只,喂以高脂饲料;2小剂量治疗组,10只,喂以高脂饲料添加蒺藜总皂苷6.3mg/kg.d.只;3大剂量治疗组10只,喂以高脂饲料添加蒺藜总皂苷12.6mg/kg.d.只;4阳性药物对照组:喂以高脂饲料添加辛伐他汀2.35mg/kg.d.只,喂养2周后,检测:1血脂;2血清IL-10、TNF-α水平。结果:与模型组相比较,大、小剂量治疗组,阳性药物对照组IL-10浓度水平升高、同时血脂、TNF-α浓度水平降低;大、小剂量治疗组比较差异均无显著性。结论:动脉粥样硬化家兔经过蒺藜总皂苷干预治疗后,血脂、血清TNF-α含量降低,同时血清IL-10含量升高,因此,推测蒺藜总皂苷是通过抑制炎症反应从而实现其抗动脉粥样硬化的作用。

Objective:To observe the effects of gross saponins from Tribulus terrestris(GSTT)on the levels of IL-10 and TNF-α in rabbits with atherosclerosis.Methods: Fifty healthy male Japanese white rabbits were used in the experiment,10 in them were randomly selected as control group fed with normal diet for 4 weeks,and the other 40 were randomly divided into four groups with 10 in each after they were fed with 4-week hyperlipidemic diet: model group with hyperlipidemic diet,low-dose GSTT group with hyperlipidemic diet and GSTT 6.3mg/kg·d,high-dose GSTT group with hyperlipidemic diet and GSTT 12.6mg/kg·d and positive control group with hyperlipidemic diet and simvastatin 2.35 mg/kg·d,respectively.After 2 weeks,the levels of lipid,IL-10 and TNF-α were determined.Resluts:As compared with model group,the levels of lipid and TNF-α was decreased in the low-dose,high-dose GSTT and positive control group.As compared with model group,the serum IL-10 level was increased significantly.Conclusion:GSTT could inhibit atherosclerosis by decreasing the levels of lipid and TNF-α and increasing the of serum IL-10 level.