苯二氮[艹卓]受体与异氟烷遗忘作用关系的实验研究

Experimental study on the relationship between benzodiazepine receptor and the isoflurane -induced amnesia in mice

目的 通过观察氟马西尼对异氟烷遗忘作用的影响,揭示异氟烷遗忘机制与苯二氮[艹卓]受体的关系.方法 将48只小鼠随机分成6组,采用避暗实验观察腹腔注射不同剂量氟马西尼（0.5、1.0 mg/kg）对异氟烷处理小鼠步入潜伏期和错误次数的影响.结果 与生理盐水组相比,氟马西尼单用组小鼠的步入潜伏期和错误次数均无明显差异（P〉0.05）;异氟烷单用或合用组步入潜伏期明显缩短（P〈0.05）,错误次数明显增加（P〈0.05 或P〈0.01）.与异氟烷组相比,两药合用组步入潜伏期和错误次数均无统计学差异（P〉0.05）.结论 氟马西尼对异氟烷遗忘作用无明显影响,提示异氟烷遗忘作用与苯二氮[艹卓]受体关系不大.

Objective To investigate the effect of flumazenil on the isoflurane - induced amnesia and to reveal the relationship between benzodiazepine receptor and the mechanism of isoflurane - induced amnesia. Methods 48 mice were randomized into six groups. The effects of intraperitoneal injection of different doses of flumazenil （0.5, 1.0 mg/ kg） on the step -through latency and error times were observed in mice treated with isoflurane （0.2 mL/kg）. Results Compared with the normal saline group, the step - through latency and error times had no statistical differences in the flumazenil groups （P 〈 0.05 ） ; the isoflurance group and the isoflurance - flumazenil combination groups showed reduced step - through latency （ P 〈 O. 05 ） and elevated error times （ P 〈 0. 01 or P 〈 0.05 ）. Compared with the isoflurance group, no statistical differences in step - through latency and error times were observed between the isoflurance group and the isoflurance - flumazenil combination groups （P 〉 0.05 ）. Conclusion Flumazenil has no significant influence on the isoflurance -induced amnesia, suggesting that the isoflurance -induced amnesia is not related to benzodiazepine receptor.