转Bar基因小麦及其杂交后代旗叶的衰老

Senescence in flag leaves of Bar-transgenic wheat and their hybrids

转Bar基因小麦具有抗除草剂的功能,是选育高产优质抗除草剂小麦品种的重要材料,Nong4是Bar基因插入在受体小麦D染色体组上的转Bar基因小麦。该研究通过测定比较转Bar基因小麦Nong4及其杂交后代各生育期旗叶的叶绿素含量、丙二醛含量、可溶性蛋白质含量以及SOD、CAT、POD等酶活性,探讨了转Bar基因小麦Nong4及其杂交后代旗叶在衰老过程中的生理变化和遗传规律。结果显示:由孕穗期至乳熟期间,转Bar基因小麦Nong4及其杂交后代旗叶的衰老在灌浆期后明显,其中杂交F1代衰老趋势较亲本转Bar基因小麦Nong4缓慢,表现出一定的中亲优势,表明转Bar基因小麦与当地优良小麦品种杂交后代的抗衰老特性较亲本转Bar基因小麦Nong4好,可以进一步选育出高产优质抗除草剂小麦品种;而F2代则没有表现出明显的优势,这可能在F2代中有基因分离现象。

Bar-transgenic wheat is an important material in the selection of high yield and quality wheat for its anti-herbicide performance.It＇s physiological change in flag leaf during senescence were studied by comparison of chlorophyll content,MDA,soluble protein content,and SOD,CAT,POD enzyme activities.The senescence of Bar-transgenic wheat Nong4 and the hybrid progeny were getting obvious after grouting,during which the senile tendency of hybrid progeny F1,a cross of Nong4 and local good wheat cultivar,was slower than the parent,Bar-transgenic wheat Nong4,and thus displayed the potential to be a good material for selection of anti-herbicide wheat cultivar.However,the inbred line F2 generation did not exhibit any superiority.