摘要
目的:探讨益气温阳化瘀法之肾消方对糖尿病肾病大鼠肾脏蛋白激酶C活性的影响。方法:单肾切除加链脲佐菌素(STZ)腹腔注射制作糖尿病肾病动物模型。分别予以中药肾消方和厄贝沙坦干预治疗16周,测定大鼠肾组织PKC活性,检测24 h尿蛋白排出量(Upro),肾脏常规染色光镜观察病理变化。结果:16周时单肾糖尿病大鼠肾组织出现肾小球硬化病理改变,Upro明显升高,细胞膜PKC活性(mPKC)明显升高,细胞浆PKC活性(cPKC)明显降低,mPKC和cPKC比值(M/C)明显升高。肾消方和厄贝沙坦干预治疗显著降低Upro,降低mP-KC和M/C,升高cPKC(P<0.01),改善肾组织病理改变。结论:肾消方对单肾切除STZ糖尿病大鼠具有肾保护作用,可通过抑制PKC的活性进而延缓DN的发生。
Objective:To investigate influence of administration of Shenxiao Decoction on protein kinase C(PKC) activity,renal function and structure in diabetic nephropathy rat.Method:Experimental diabetic nephropathy model was induced by nephrectomy combined with streptozotocin(STZ) injection in sprague-dawley rat.Shenxiao Decoction and Irbesartan were orally administrated respectively for 16 weeks and PKC activity was measured.24h urine protein excretion(Upro) and renal pathological changes were observed.Result:In 16th week,diabetic nephrectomized rats developed proteinuria,glomerulosclerosis,increased membrane PKC activity(mPKC),decreased cytosol PKC(cPKC),and increased ratio of mPKC and cPKC(M/C).Administration of Shenxiao Decoction or Irbesartan led to a reduction in proteinuria,structural injury,mPKC and M/C,and a recovery in cPKC.Conclusion:Shenxiao Decoction possesses a renoprotective effect on diabetic nephrectomized rat,at least partially via the inhibition of PKC activation in renal cortex.
出处
《中华中医药学刊》
CAS
2011年第11期2465-2467,I0002,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药管理局资助项目(2008Z005)
杭州市卫生局重点资助项目(2008YA026)
关键词
糖尿病肾病
蛋白激酶C
肾消方
厄贝沙坦
protein kinase C
diabetic nephropathy
Shenxiao Decoction
Irbesartan