胆胃舒颗粒预防胆囊结石及对胆囊收缩素受体(CCK-AR)基因表达的影响

Experimental Study on Danweishu Granule in the Prevention of Gallstone Recurrence after Stone Removement and Gallstone CCK-AR Gene Expression

目的:观察利胆健脾方胆胃舒颗粒对胆囊结石的预防作用及其作用机制。方法:实验用短毛雄性豚鼠90只,随机分为3组,每组30只,空白组:喂养普通饲料每天40g/只;模型组:喂养胆固醇结石诱石饲料40g/天/只;治疗组喂养胆固醇诱石饲料40g/天/只,并胆胃舒颗粒溶液灌胃2mL(含300mg胆胃舒颗粒)。实验2个月后观察胆囊内结石情况、胆囊收缩功能、及胆囊、胃窦平滑肌CCK-AR最大结合容量;结果:胆囊结石情况:空白组3.33%(1/30);模型组92.59%(25/27);治疗组:10.71%(3/28)。胆囊收缩功能:空白组为66.83%±5.34%;模型组43.06%±4.27%;治疗组67.93%±6.82%;胆囊平滑肌CCK-AR基因表达:空白组0.862±0.112;模型组0.521±0.083;治疗组0.792±0.098。结论:利胆健脾法可降低胆囊结石的形成,其作用机制可能通过提高胆囊CCK-AR基因表达,从而加强胆囊收缩功能,促使胆汁流动,防止胆囊结石的发生。

Objective： To explore the mechanisms of Danweishu granule of Lidan Jiaupi Prescription in the prevention of gallstone recurrence after stone removement and gallstone CCK-AR gene expression.Method：90 guinea pig were randomly divided into control group（fed with normal diet,n=30）,model group（fed with lithogenic diet,n=30） and treatment group（fed with lithogenic diet plus Fangshi capsule,n=30）.After two months feeding,the animals were sacrificed and sampled,the expression of CCK-A receptor mRNA in gallbladder tissue was subjected to RT-PCR assay.At the sametime,gallbladder contraction rate and the rates of gallstone formation in each group were observed.Results：Compared with the model group,increase of gallbladder contraction rate and the expression of CCK-A receptor mRNA（P0.05） were found in the treated group.Conclusion：Lidan Jianpi Therapy can prevent recurrence of gallstone,its mechanism is possibly correlated with increasing CCK-AR mRNA expression in gallbladder tissue.