锻炼能提高动脉硬化患者行动能力

Effects of protocatechuic acid on the mRNA expression of three transport proteins in HepG2.2.15 cell and rat primary hepatocyte

如今老年人患有下肢动脉硬化闭塞症的情况越来越常见，这会导致行动不便和生活难以自理。美国西北大学范伯格医学院的一项临床试验表明，在专家指导下的肢体锻炼，能够有效提高患者的行动能力。

Objective： To study the effects of protocatechuic acid（PA） on the mRNA expression of transporters P-gp,Oatp1A1 and Oct1 in HepG2.2.15 cell and rat primary hepatocyte.Methods： HepG2.2.15 cells and rat primary hepatocytes were treated in vitro by different concentration of PA respectively,the mRNA expression levels of P-gp,Oatp1A1 and Oct1 were detected by real-time fluorescence quantitative PCR.Reaults： After HepG2.2.15 cells were treated by PA for 48h,low concentration of PA（0.5μg/mL） significantly decreased the mRNA expression of P-gp and Oatp1A1,whereas a reverse changes were observed at the high concentration of PA（50μg/mL）.Meanwhile,PA at low,medium and high concentration caused marked increase of Oct1 mRNA expression.For rat primary hepatecytes treated by PA for 48h,low concentration of PA caused significant decrease of the mRNA expression of OatplAl and Oct1,whereas high concentration of PA caused marked increase of the mRNA expression of Oatp1A1 and Oct1.Conclusion： The effects of PA on the mRNA expression of P-gp,Oatp1A1 and Oct1 in HepG2.2.15 cell and rat primary hepatocyte suggested that the drug-drug interaction between PA and other drugs,which were transported by the above transporters,should occurred.