水稻磷脂酶A活性测定方法的研究

Studies on the Measurement of Phospholipase A Activity in Rice

为了优化水稻磷脂酶A活性测定方法,研究了多种因素对水稻磷脂酶A活性的酸碱滴定测定方法的影响。结果表明水稻磷脂酶A活性酸碱滴定法测定的适宜条件为:起始pH8.0,反应温度40℃,20.0 mL底物,4.0 mL酶的粗提液,提取缓冲液的体积与叶片样品重量的比值(mL/g)为2.5,反应时间7 h。

To optimize the determination conditions for phospholipase A activity,the effects of several factors on the acid base titration assay of phospholipase A activity were investigated.The results showed that the best conditions for the assay were as follows： initial pH 8.0,reaction temperature 40 ℃,substrate volume 20.0 mL,enzyme extract volume 4.0 mL,extraction buffer volume/sample weight（mL/g） 2.5,and reaction time 7h.